Fischer carbene complex anchored on glass or silicon surface using a Cu-free 'click' reaction allows facile and swift covalent grafting of protein molecules like Bovine Serum Albumin (BSA).
A Fischer carbene complex was grafted onto self-assembled monolayers (SAMs) on gold or glass by a copper-free "click" reaction. Pendant lysine residues of protein A obtained from Staphylococcus aureus rapidly reacted with the electrophilic metal complex on SAM effecting a covalent attachment of protein A with the surface. The protein A coated surface further led to bioaffinity immobilization of rabbit IgG in an oriented manner, a feature that also permits its purification from rabbit serum. Rabbit IgG could be removed from protein A coated surface by pH adjustment. The regenerated protein A surface was reused three times without loss of activity.
Nanoparticulate palladium (Pd np -2) supported on polyethylene glycol-functionalized silica gel is an excellent heterogeneous catalytic system for Suzuki, Sonogashira and Stille cross-coupling reactions that afford products in high yield. The immobilized palladium nanoparticles were readily prepared by reaction of modified silica gel (1) with potassium tetrachloropalladate(II) in the presence of an acyl metal salt of a Fischer carbene complex. The nanoparticles were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The catalyst (Pd np -2) is thermally stable, insensitive to oxygen, and recyclable. The catalyst has been used for five cycles without noticeable loss of catalytic activity.
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