No abstract

No abstract

No abstract

Background: To investigate the effects of tannic acid (TA) use on ovarian folliculogenesis, p53 expression, and serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (T) levels in rats with polycystic ovary syndrome (PCOS) model. Materials and Methods:A total of 18 Wistar female rats were used and divided into 3 experimental groups. Group 1 (G1, control), group 2 (G2, PCOS), group 3 (G3, PCOS+TA). Rats were induced with letrozole for 21 days to form a PCOS model. After 21 days, TA (40 mg/kg) was given by gavage for 10 days and the rats were sacrificed on the 10 th day. PCOS formation was evaluated by daily estrous cycle follow-up. Hematoxylin & eosin and p53 immunohistochemical staining was performed on ovaries. Serum FSH, LH, and T levels were determined by ELISA. Data were analyzed with the One-Way ANOVA test and Kruskal-Wallis H test. P<0.05 was considered statistically significant. Results:The number of cystic follicles was significantly increased in the PCOS group compared to the TA treatment group (p<0.05). The number of primary follicles was significantly increased in the TA treatment group (p<0.001). No significant change was observed in the number of primordial, secondary, and Graaf follicles between the experimental groups. A significant increase in LH and T was observed in the PCOS group (p<0.05). The increase in LH has significantly decreased TA administered rats (p<0.05). Although it was not significant, serum FSH level was increased in the PCOS+TA group. No immunoreactivity was detected in p53 staining in experimental groups. Conclusion:TA can decrease cystic follicle formation and increase primary follicle formation in PCOS. Also, it can regulate the hormonal expression of serum LH, FSH, and T in PCOS-modeled rats.

Background: To compare the changes caused by low and high dose rate radiotherapy in the bladder tissue of rats and to examine the effect of melatonin on the bladder tissue.Materials and Methods: 40 adult rats were randomly divided into five groups with 8 rats in each group. Radiotherapy and melatonin treatment were not applied to group 1 (G1) control group rats. A single dose of 8 Gy and 400 MU/min radiotherapy was applied to G2 and G3 group rats. A single dose of 8 Gy and 1400 MU/min radiotherapy was applied to G4 and G5 group rats. G3 and G5 group (treated groups) rats were given 50 mg/kg melatonin intraperitoneally 15 minutes before the radiotherapy application. Rats were sacrificed under anesthesia and bladder tissues were removed. Histopathological examination was performed on the samples stained with hematoxylin eosin and toluidine blue.Results: Number of mast cells was increased in G2 and G4 (p<0.01). In addition, edema and vascular congestion were observed in these groups. In G3 and G5, acute phase markers decreased compared to radiotherapy received groups. Conclusion:Bladder tissue degradation was observed in G2 and G4 compared to the control group. However, there was no difference in bladder tissue between the groups given two different doses. The acute phase markers of edema, number of mast cells, inflammatory cell infiltration, and vascular congestion in the bladder tissue of the groups administered melatonin decreased.

Purpose: The aim of this study was to investigate the effects of vitamin E (Vit E) and vitamin C (Vit C) on oxidant-antioxidant system markers, ovarian follicle reserves, and surface epithelium in autologous intraperitoneal ovarian transplantation in rats.Materials and Methods: 20 adult female Wistar Albino were randomly divided into four groups. Group 1 (n = 5), the control group, only had their abdomens opened and closed. Group 2 (n = 5): underwent an ovarian transplantation. Group 3 (n = 5) received 20 mg/kg of intraperitoneal (IP) Vit E 15 minutes before an ovarian transplantation. Group 4 (n = 5) received 50 mg/kg of IP Vit C that was administered 15 minutes before an ovarian transplantation.Vaginal cytology was performed to monitor the oestrus phase. Biochemically, tissue and serum malondialdehyde levels and erythrocyte superoxide dismutase (SOD) levels were measured. Histopathologically, the number dysplastic changes in the ovarian surface epithelium were examined. Results: Dysplastic changes in the surface epithelium of Group 2 were found to be significantly higher than in Group 1 and 4 (p < 0.02). In Group 2, the ovarian follicle reserves were significantly lower than in other groups (p < 0.02). In addition, a significant decrease in SOD levels was found in Group 2 compared to other groups (p < 0.02). Conclusion: The study showed that Vit E and Vit C in autologous intraperitoneal ovarian transplantation preserved the ovarian follicle reserve. Vit C was found to be more effective than Vit E.

Melatonin is a hormone that exhibits many bioactivities such as antioxidant, anti-inflammatory, anti-carcinogenic, anti-diabetic, neuroprotective, and anti-aging activity. In addition, melatonin has been used to strengthen the effect of drugs or agents used for treatment in many different disease models such as cancer, tumor, bacterial infection, autoimmune diseases, and gastrointestinal diseases. According to recent studies, curcumin is used as a pigment and powerful antioxidant and anti-inflammatory agent with therapeutic potential against various cancers. Melatonin and curcumin have been used in different models of disease or injury: such as Alzheimer's disease treatment, testicular tissue preservation, bladder cancer treatment, gastric mucosal damage, and prevention of nephrotoxicity. This review covers published literature studies on the effects of comparison or combined use of melatonin and curcumin as anticancer and antioxidant agents and it reveals whether melatonin potentiates the effect of curcumin when used together.

No abstract