Comparison of melatonin, oxytetracycline, and N-acetylcysteine pre-treatments in autologous intraperitoneal ovarian transplantation in rats

Sapmaz, Tansel; Sevgin, Kubra; Topkaraoglu, Sude; Tekayev, Muhammetnur; Aktaş, Selman; Çoşkun, Gülfidan; Polat, Sait; Sapmaz, Ekrem; İrkörücü, Oktay

doi:10.1016/j.bbrc.2022.03.065

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…Previous studies have shown that melatonin exerts a powerful regulatory effect on the female reproductive system 8,9 . In the study, melatonin effectively increased the antioxidant capacity of whole transplanted ovaries, preserving more follicular reserve, and maintaining endocrine hormone secretion in the melatonin groups, which was in accordance with previous ndings [19][20][21][22][23][24][25][26][27] . Several studies have found that melatonin is also a potent immunomodulator that plays a role in humoral and cellular immune pathways after organ transplantation [28][29][30] .…”

Section: Discussionsupporting

confidence: 90%

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Melatonin protects ovarian function in whole cryopreserved rat ovarian transplantation via the MT1/Nrf2/ARE pathway

Xie

Hua

Zhang

et al. 2023

Preprint

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Background: Whole ovarian transplantation has the potential to restore fertility in cancer patients, but ovarian ischemia-reperfusion injury following transplantation causes decreased graft function. Melatonin protects against antioxidant damage and has anti-inflammatory effects, but its effects in whole ovarian transplantation have not been investigated. Objective:This study was aimed to verify the beneficial antioxidant and anti-inflammatory effects of melatonin in whole ovarian transplantation. Methods: The cryopreserved whole ovaries were allotransplanted in LEWIS rats. Forty rats were randomly divided into 8 groups: control group, sham surgery group, saline group; low-dose (25 mg/kg) melatonin group; high-dose (50 mg/kg) melatonin group; melatonin (50 mg/kg) + ML385 group; melatonin (50 mg/kg) + luzindole group, and melatonin+ 4P-PDOT group. The estrous cycle recovery was evaluated by vaginal exfoliative cell monitoring and serum hormone. Follicle morphology was observed by HE. The levels of eoxidative stress factors, antioxidant factors, and inflammatory factors in both serum and ovarian tissues were measured by ELISA, RT-qPCR, western blot and fenton detection. RT-qPCR, western blot and immunofluorescence assays were used to measure the levels of MT1 and Nrf2. Results: The rats in high-dose and low-dose melatonin groups recovered estrous cycle faster and lost fewer follicles, and the serum endocrine hormone levels were close to normal. The serum and ovarian tissue antioxidant capacity were significant higher, while the levels of inflammatory factors were significant lower in the high-dose and low-dose melatonin groups. In addition, the melatonin receptor MT1 was found to be involved in antioxidant and anti-inflammatory processes. Melatonin also triggered the Nrf2/ARE pathway activity via receptor MT1. Blocking Nrf2 or MT1 receptors could eliminate the beneficial effects of melatonin on whole transplanted ovaries. These findings suggest that melatonin can attenuate oxidative stress injury and inflammatory responses in whole transplanted ovaries via the MT1/Nrf2/ARE signaling pathway, thereby effectively protecting whole transplanted ovarian function.

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Section: Discussionsupporting

confidence: 90%

“…Many previous experiments have been successfully performed using human or animal ovarian cortical slices for transplantation [19][20][21][22][23][24][25][26][27] . However, ovarian tissue without vasculature is exposed to an ischemic period of several days.…”

Section: Discussionmentioning

confidence: 99%

Melatonin protects ovarian function in whole cryopreserved rat ovarian transplantation via the MT1/Nrf2/ARE pathway

Xie

Hua

Zhang

et al. 2023

Preprint

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“…In non-obese diabetic mice, high administration of melatonin predominantly enhanced survival of islet graft as it inhibited T cell proliferation and increased IL-10 cell population (Lin et al 2009 ). In a rat model with ovary transplantation, the melatonin administration depicted immunosuppressive property (Sapmaz et al 2003 ). A comparable research study of ovarian grafts in humans treated with melatonin showed reduced apoptosis (Friedman et al 2012 ).…”

Section: Melatonin In Transplantationmentioning

confidence: 99%

Melatonin and Health: Insights of Melatonin Action, Biological Functions, and Associated Disorders

et al. 2023

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Melatonin is ubiquitous molecule with wide distribution in nature and is produced by many living organisms. In human beings, pineal gland is the major site for melatonin production and to lesser extent by retina, lymphocytes, bone marrow, gastrointestinal tract, and thymus. Melatonin as a neurohormone is released into circulation wherein it penetrates all tissues of the body. Melatonin synthesis and secretion is supressed by light and enhanced by dark. Melatonin mostly exerts its effect through different pathways with melatonin receptor 1 (MT1) and melatonin receptor 2 (MT2) being the predominant type of receptor that are mainly expressed by many mammalian organs. Melatonin helps to regulate sleep patterns and circadian rhythms. In addition, melatonin acts as an antioxidant and scavenges excessive free radicals generated in the body by anti-excitatory and anti-inflammatory properties. A multiple array of other functions are displayed by melatonin that include oncostatic, hypnotic, immune regulation, reproduction, puberty timing, mood disorders, and transplantation. Deficiencies in the production or synthesis of melatonin have been found to be associated with onset of many disorders like breast cancer and neurodegenerative disorders. Melatonin could be used as potential analgesic drug in diseases associated with pain and it has quite promising role there. In the past century, a growing interest has been developed regarding the wide use of melatonin in treating various diseases like inflammatory, gastrointestinal, cancer, mood disorders, and others. Several melatonin agonists have been synthesized and are widely used in disease treatment. In this review, an effort has been made to describe the biochemistry of melatonin along with its therapeutic potential in various diseases of humans. Graphical Abstract

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Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods

Silva,

Rodrigues,

Ribeiro

et al. 2024

Cryobiology

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Comparison of melatonin, oxytetracycline, and N-acetylcysteine pre-treatments in autologous intraperitoneal ovarian transplantation in rats

Cited by 6 publications

References 24 publications

Melatonin protects ovarian function in whole cryopreserved rat ovarian transplantation via the MT1/Nrf2/ARE pathway

Melatonin protects ovarian function in whole cryopreserved rat ovarian transplantation via the MT1/Nrf2/ARE pathway

Melatonin and Health: Insights of Melatonin Action, Biological Functions, and Associated Disorders

Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods

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