High blood cholesterol level can cause atherosclerosis which is potentially cause coronary heart disease. Pindang patin is one of the favourite foods in Palembang which contain omega 3 fatty acids that can decrease blood cholesterol level. The aim of this study was to see the effect of pindang patin intake on total cholesterol and LDL. The type of this study was laboratory experimental using a pre-post-test randomized design with a control group in male mice (Mus musculus L.). The mice were given the high-fat diet by using egg yolk as much as 20% of body weight. The samples were divided into 5 groups: Negative Control, Positive Control, Dose I (0.26 ml/20 grams of body weight), Dose II (0.52 ml/20 grams of body weight) and Dose III (1.04 ml/20 grams of body weight). Blood samples were examined by using a spectrophotometer. Dose II and Dose III give the effect in decreasing the blood level of cholesterol in male mice, and Dose III give the effect in decreasing the blood level of LDL of male mice. Pindang patin can decrease the blood total cholesterol and LDL levels of male mice, and the best dose is in 1.04 ml/20 grams of body weight.
Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow's milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow's milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAEcellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a-SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after β β β β β-mercaptoethanol treatment. In contrary, the folate binding didn need Ca 2+ ion, as indicated by EDTA test which gave the same result as control.
Background<br />Smoking is the act of introducing toxic substances into the body. Cigarette smoke contains chemicals that may cause several disorders, including cardiovascular disease, cancer, and chronic obstructive pulmonary disease. Toxic substances in cigarette smoke have the potential to increase free radicals, malondialdehyde (MDA) levels and to decrease endogenous antioxidant (glutathione/GSH) levels. This study aims to determine the relationship of smoking with plasma GSH and MDA levels in medical students.<br /><br />Methods<br />This study was analytical observational with cross-sectional approach. The study was conducted from April to December 2015. The subjects in this study were medical students, consisting of 20 smokers and 20 nonsmokers. Plasma GSH and MDA levels were determined biochemically using Sigma GSH Assay Kit and Sigma MDA Assay Kit. Data was analyzed using the independent t test. <br /><br />Results<br />The results showed that there was no significant difference between mean GSH in smokers (1.74 ± 0.91 mmol/L) and nonsmokers (2.42 ± 1.19 µmol/L) (p=0.441). Mean smokers MDA level of 2.06 ± 1.39 nmol/mL was not significantly different compared with mean nonsmokers MDA level (1.32 ± 0.90 nmol/mL) (p=0.092).<br /><br />Conclusions<br />Smoking tends to decrease plasma GSH levels and increase plasma MDA levels in medical students. Smoking history could be evidence of oxidative stress and an impaired oxidant defense system. In particular, young smokers should quit promptly before health problems arise, so as to have the optimal benefits of cessation.
Potensi Antioksidan Ekstrak Biji Duku (Lansium Domesticum Corr. Analisis uji ANOVA menunjukkan ada satu kelompok yang memiliki perbedaan rata-rata baik kadar GSH (p=0,00) maupun kadar MDA (p=0,00). Analisis uji post hoc terhadap kadar GSH dan MDA menunjukkan hanya kelompok dosis 100 mg/kgbb yang memiliki perbedaan yang bermakna (p=0,00). Dengan demikian, ekstrak biji duku (Lansium domesticum Corr.) memiliki potensi antioksidan dan optimum pada dosis 100 mg/kgbb.Kata Kunci: antioksidan, ekstrak biji duku, GSH, MDA ABSTRACTLansium domesticum Corr. is a typical plant of Indonesia. The seeds have a lot of useful compounds, such as flavonoids. Flavonoids are a natural compound that can act as antioxidants. The aim of this study was to determine the antioxidant potential of Lansium domesticum Corr. seed extract. This study was an experimental research with posttest control group design. The subjects were 32 white male rats. Rats were randomly divided into 4 groups: negative control group was given suspension of Na CMC 1%, dose group I, II and III were given Lansium domesticum Corr. seed extract with each dose of 100mg/kg, 200 mg/kg, and 300 mg/kg. Observation of the subject's response was done by measuring the levels of glutathione (GSH) and malondialdehyde (MDA). Analysis ANOVA test showed that there was a group that had an average difference both GSH levels (p=0.00) and MDA levels (p=0.00). Post hoc test analysis for GSH levels and MDA levels showed that only a dose of 100 mg/kg which have significant differences (p=0.00). Thus, Lansium domesticum Corr. seed extract has antioxidant potential and optimum dose of 100 mg/kg.
Biji pepaya dapat bersifat sebagai antifertilitas. Penelitian ini dilakukan untuk mengetahui senyawa dan mekanisme antifertilitas fraksi biji pepaya pada tikus jantan dari berat vesikula seminalis dan prostat serta jumlah spermatosit primer dan spermatid. Jenis penelitian eksperimental secara in vivodengan rancangan acak lengkap. Hewan uji adalah tikus jantan (Rattus norvegicusL.) galur Sprague Dawley usia 60 hari dengan berat badan 200-210 g. Dosis pemberian 100mg/kg bb selama 24 hari. Senyawa yang dikandung oleh fraksi didapat melalui uji fitokimia. Hasil penelitian memperlihatkan senyawa yang ditemukan pada fraksi nHeksan adalah steroid, terpenoid, saponin, flavonoid. Fraksi etil asetat terdapat senyawa steroid, terpenoid dan flavonoid. Fraksi etanol air mengandung senyawa terpenoid dan flavonoid. Berat vesikula seminalis dan prostat meningkat dibandingkan kontrol. Rerata jumlah spermatosit primer dan spermatid menurun secara nyata pada semua fraksi namun penurunan terendah pada fraksi nHeksan. Dapat disimpulkan mekanisme antifertilitas fraksi biji papaya adalah melalui penurunan rerata jumlah spermatosit primer dan spermatid.
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