Objective: The purpose of this study was to determine the effect of extra virgin olive oil (EVOO) and rice bran oil (RBO) on glycemic control and lipid profiles in patients with type-2 diabetes mellitus (T2DM). Methods: Ten patients with T2DM received 15 ml/day of EVOO or RBO. Levels of fasting blood glucose (FBG), postprandial blood glucose (PBG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), and triglycerides (TGs) were measured. RBO or EVOO was administered for 4 consecutive weeks. During a 2-week interval, the treatment was not administered. After this washout period, a crossover design was implemented by exchanging EVOO supplementation with RBO supplementation and vice versa for 4 consecutive weeks. Results: Changes in levels of FBG, PBG, TC, LDL-C, and TGs were not significantly different in the two groups. However, significantly decreased the levels of HDL-C were observed in both groups. Conclusion: RBO and EVOO had no significant influence on levels of FBG or PBG.
Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow's milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow's milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAEcellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a-SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after β β β β β-mercaptoethanol treatment. In contrary, the folate binding didn need Ca 2+ ion, as indicated by EDTA test which gave the same result as control.
Materials Mung Bean Thiamine Binding Protein (MBTBP) used was the result of post-affinity chromatography with levels of 1446 μg/mL that had been identified and analyzed. KH 2 PO 4 , Na 2 HPO 4 , NaCl, NaOH, BSA, sodium EDTA, iodoacetate, hydrogen peroxide, β-mercaptoethanol, HgCl 2 , AgNO 3 , thiamine, buffer sulphate, dialysis tubing cellulose membrane was purchased from Sigma-Aldrich. Methods Protein assay Concentration of protein was determined by the method of Warburg-Christian using bovine serum albumin as the standard. 5 Effect of temperature and storage time on MBTBP stability MBTBP powder was known to be concentrated and diluted to a concentration of 250 μg/mL in aliquoting and stored at-20°C, 4°C and 37°C with storage time of 3 days, 7 days, 14 days and 30 days. 6,7 Furthermore, protein levels were measured using a standard BSA curve.
Introduction: Adult individuals in Indonesia showed changes in diet and lack of physical activity, therefore increasing the risk of hypercholesterolemia and obesity. One of nutritional therapy for hypercholesterolemia is rice bran oil, which contains active substances that works synergistically in controlling lipid profile. The substances are gamma-orzanol, phytosterols, and vitamin E isomers (tocotrienol and tocopherol).Methods: A clinical trial was conducted for 4 weeks in City Hall of Jakarta on 20 males, age 19–55 years old, with mild-moderate hypercholesterolemia, to assess lipid profile improvement of the supplementation of rice bran oil 45 ml/days compared to 15 ml/days without changing eating patterns.Results: After 4 weeks of intervention, there was significant difference in total cholesterol level between both groups (p = 0.049). In the group that received 45 ml/days of rice bran oil, total cholesterol level decreased 14%, and in the group of 15 ml/days total cholesterol level decreased 7.8%. The reduction of LDL and triglycerides and the increase of HDL were not significantly different between both groups (p >0,05). There was no significant changes of body weight in both groups.Conclusion: This study showed that consumption of 45 ml/days rice bran oil led to better improvements in lipid profiles compare to 15 ml/days. It has been demonstrated that gamma oryzanol and plant sterols in rice bran oil have the capability to remove cholesterol from bile salt micelles, thus decreasing cholesterol absorption in intestine.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.