Background
Diagnosis of tuberculosis (TB) in the era of technological sophistication requires accuracy and speed to provide as much information as possible so that TB treatment can be carried out quickly and precisely. New studies have also begun to be carried out to diagnose TB, one of which is by examining genes, either by looking at polymorphisms, mutations, or expressions. Several previous studies have confirmed the association of MC3R and TB genes with polymorphisms; MC3R is a gene that participates in the regulation of the inflammatory process and is also found in macrophages; therefore, we tried to analyze gene expression in the active TB group, household contacts, and healthy controls for looked at the differences between the three groups and confirmed the correlation of MC3R with TB by seeing which group's gene expression increased the most expression of the three groups so that the results can be considered as a TB diagnostic biomarker in the future.
Methods
This study included 122 people, 49 patients with confirmed TB, 46 close relatives of patients, and 27 healthy controls. This study used a real-time PCR technique to analyze MC3R gene expression in the three groups, and all data were analyzed using Bio-Rad CFXTM software version 3.1 and one-way ANOVA using SPSS 21.0.
Results
The value of MC3R gene expression in the active TB group increased 3.6-fold in the healthy group (p = 0.143), and that of gene expression in the healthy control group increased 1.09-fold in the healthy group (p = 0.007).
Conclusion
There is a relationship between MC3R and TB based on the results of gene expression analysis that increased in the active TB group compared to the household contact group and healthy controls.
Kuliah Kerja Lapang Plus (KKLP) merupakan suatu kegiatan perkuliahan yang bersifat intrakurikuler dalam bentuk pengabdian pada masyarakat yang dilakukan oleh masyarakat dalam waktu tertentu. oleh karena, sifatnya intraku rikuler sehingga setiap mahasiswa yang akan menyelesaikan studi di perguruan tinggi pada suatu program strata satu (SI) harus mengikuti KKLP.
The research on early identification of bacterial from juveniles top shell (Trochus niloticus L.) and giant clam (Tridacna squamosa Linn.) was conducted at Unhas’s hatchery at Barrang Lompo island during August-October 2006. Bacteriology test was done at Microbiology Laboratory of Biology Department, Math and Science Faculty, Hasanuddin University, Makassar. The quantitative test was done using Most Probable Number (MPN) and Standard Plate Count (SPC) methods. While the qualitative test included bacteria colony observation, macroscopic, microscopic, and biochemical test. Macroscopic observation was done by assessing the form, elevation, color, ridge, and inner structure of bacterial colony. Microscopic observation was conducted by using Gram and spora stain. The result of MPN method shows the average total bacteria for juveniles top shell is 17.5 x 102 cell/ml and for giant clam is 6.65 x 102 cell/ml, while SPC results indicate the average total bacteria for juveniles top shell is 4.8 x 105 cell/ml and for giant clam is 3.0 x 105 cell/ml, respectively. The result of biochemical test identifies 5 genera of bacteria such as Micrococcus, Bacillus, Streptomyces, Escherichia and Enterobacter.
Kuliah Kerja Lapang Plus (KKLP) merupakan suatu kegiatan perkuliahan yang bersifat intrakurikuler dalam bentuk pengabdian pada masyarakat yang dilakukan oleh masyarakat dalam waktu tertentu. oleh karena, sifatnya intrakurikuler sehingga setiap mahasiswa yang akan menyelesaikan studi di perguruan tinggi pada suatu program strata satu (SI) harus mengikuti KKLP.
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