BACKGROUND: Selenium (Se) is a needed trace element for animals and humans. Many fungi have effective mechanisms to acquire, transform and accumulate Se in organic form. In this study, the effects of inorganic Se (sodium selenite) on the medicinal fungus Inonotus hispidus was investigated. RESULTS: Inonotus hispidus was capable of tolerating up to 3.85 mmol L −1 selenite, at which ∼85% growth inhibition was seen, with 50% growth inhibition occurring at ∼1 mmol L −1 selenite. Growth in 0.29 mmol L −1 Se resulted in I. hispidus mycelium with 115 times higher Se levels compared to growth in standard media, and an organic Se content of 86% to total Se content. The influence of Se accumulation on morphological features of I. hispidus were examined by microscopic and scanning electron microscopic observation. These data revealed significant shrinkage and deformations of I. hispidus hyphae with decreased branching and collapse of clamp connections under higher Se stress. However, conidial production in I. hispidus increased dramatically. The influence of Se on mycelial growth could be recovered by reinoculation in standard media. Se accumulation had only minimal impacts on the yield of the potential selenocompounds such as amino acids, proteins and polysaccharides. By contrast, Se-enriched I. hispidus mycelium was of higher quality due to reduction in crude fat and total ash contents.CONCLUSIONS: These data provide basic and applied information on the feasibility of producing selenized I. hispidus as an enriched and better quality product.
Morchella importuna is a highly priced edible and medicinal mushroom. Crop rotation is an important management technique to improve soil health. In this study, the morphological characteristics, chemical composition, and nutritional quality of the M. importuna fruitbody under five different rotation systems (named RSA to RSE) were investigated. The results showed that the fruitbodies of M. importuna in rotation system C (RSC, Rice–Pea–M. importuna rotation) grew best (with the highest yield of 6804.90 kg/hm2) and were of higher quality, which showed significant increases in crude protein (37.32 g/100 g) and decreases in crude fat (4.04 g/100 g), crude fiber (10.06 g/100 g), and total ash (9.32 g/100 g). The heavy metal contents (Pb, Cd, and Hg) in the fruitbodies from all rotation systems were rare or none, which meets the standards of the Chinese Green Food Standard and the maximum limit in foodstuffs of the European Union Standard. In addition, the free amino acid compositions of morel under different rotation systems were analyzed. The ratio of essential amino acids to total amino acids (EAA/TAA) was highest in RSC (37.11%). The tested morels were abundant in umami, sweet, aromatic, and medicinal amino acids (UAA, SAA, AAA and MAA). Combining amino acid score, chemical score, and other nutrition indexes, RSC had the best impact on the yield and quality of morel. Our results demonstrate the feasibility and effectiveness of the cultivation model of rotating “Rice–Vegetables–Fungi” for the production of M. importuna in a way that develops high-quality agriculture.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.