Abstract. To date, only a few studies have investigated the potential of coal fly ash particles to trigger heterogeneous ice nucleation in cloud droplets. The presented measurements aim at expanding the sparse dataset and improving process understanding of how physicochemical particle properties can influence the freezing behavior of coal fly ash particles immersed in water. Firstly, immersion freezing measurements were performed with two single particle techniques, i.e., the Leipzig Aerosol Cloud Interaction Simulator (LACIS) and the SPectrometer for Ice Nuclei (SPIN). The effect of suspension time on the efficiency of the coal fly ash particles when immersed in a cloud droplet is analyzed based on the different residence times of the two instruments and employing both dry and wet particle generation. Secondly, two cold-stage setups, one using microliter sized droplets (Leipzig Ice Nucleation Array) and one using nanoliter sized droplets (WeIzmann Supercooled Droplets Observation on Microarray setup) were applied. We found that coal fly ash particles are comparable to mineral dust in their immersion freezing behavior when being dry generated. However, a significant decrease in immersion freezing efficiency was observed during experiments with wet-generated particles in LACIS and SPIN. The efficiency of wet-generated particles is in agreement with the cold-stage measurements. In order to understand the reason behind the deactivation, a series of chemical composition, morphology, and crystallography analyses (single particle mass spectrometry, scanning electron microscopy coupled with energy dispersive X-ray microanalysis, X-ray diffraction analysis) were performed with dry- and wet-generated particles. From these investigations, we conclude that anhydrous CaSO4 and CaO – which, if investigated in pure form, show the same qualitative immersion freezing behavior as observed for dry-generated coal fly ash particles – contribute to triggering heterogeneous ice nucleation at the particle–water interface. The observed deactivation in contact with water is related to changes in the particle surface properties which are potentially caused by hydration of CaSO4 and CaO. The contribution of coal fly ash to the ambient population of ice-nucleating particles therefore depends on whether and for how long particles are immersed in cloud droplets.

There is a plausible association between shift work and cardiovascular disease (CVD), which may be due to disruption of the circadian rhythm causing hormonal changes and metabolic disturbances, resulting in high blood pressure, atherosclerosis, diabetes, and being overweight. However, few studies have investigated the association between several consecutive long work shifts, including night shifts, and risk factors for developing CVD. Moreover, knowledge is lacking on factors that may modify or enhance this suggested relationship. The study period is planned from the third quarter of 2018 to the fourth quarter of 2021, and will involve 125 industrial employees at two Norwegian enterprises producing insulation. The work schedule is either rotating shiftwork (morning, evening, night) or regular day work. At baseline, we will measure blood parameters, including markers of inflammation, lipids, and glycosylated hemoglobin. We will also collect measures of blood pressure, resting heart rate, arterial stiffness, carotid intima-media thickness, and aerobic fitness. At the end of baseline data collection, a subgroup will undergo a supervised high-intensity interval training intervention for eight weeks, initiated by the Occupational Health Service. At one-year follow-up, we repeat baseline measures with added measures of heart rate variability and additional five weeks monitoring of sleep and physical activity, and assessment of respirable dust. At the two year follow-up, we will measure CVD risk factors before and after a planned three-month shutdown in one of the studied plants. We will also assess respirable dust, monitor sleep, and compile a one-year retrospective detailed overview of working hours. A final data collection, similar to the one at baseline, will be carried out after three years. We will use a comprehensive set of methods to identify the effects of shift work with long working hours and night shifts on cardiovascular health. This will provide new knowledge on the association between early manifestations of CVD and occupational exposure to shift work. Further, we can study whether work organization such as extensive overtime, sleep loss, and dust exposure have detrimental effects, and if a three-month cease in shift work or increased physical activity will modify early manifestations of CVD.

Abstract. The mineralogy and mixing state of dust particles originating from the African continent influences climate and marine ecosystems in the North Atlantic due to its effect on radiation, cloud properties and biogeochemical cycling. However, these processes are difficult to constrain because of large temporal and spatial variability, and the lack of in situ measurements of dust properties at all stages of the dust cycle. This lack of measurements is in part due to the remoteness of potential source areas (PSAs) and transport pathways but also because of the lack of an efficient method to report the mineralogy and mixing state of single particles with a time resolution comparable to atmospheric processes, which may last a few hours or less. Measurements are equally challenging in laboratory simulations where dust particles need to be isolated and characterised in low numbers whilst conditions are dynamically controlled and monitored in real time. This is particularly important in controlled expansion cloud chambers (CECCs) where ice-nucleating properties of suspended dust samples are studied in cold and mixed phase cloud conditions. In this work, the mineralogy and mixing state of the fine fraction (<2.5 µm) in laboratory-suspended dust from PSAs in north Africa were made using novel techniques with online single-particle mass spectrometry (SPMS) and traditional offline scanning electron microscopy (SEM). A regional difference in mineralogy was detected, with material sourced from Morocco containing a high number fraction of illite-like particles in contrast to Sahelian material which contains potassium- and sodium-depleted clay minerals like kaolinite. Single-particle mixing state had a much greater local variation than mineralogy, particularly with respect to organic–biological content. Applying the same methods to ambient measurement of transported dust in the marine boundary layer at Cabo Verde in the remote North Atlantic enabled the number fractions of illite/smectite clay mineral (ISCM), non-ISCM and calcium-containing particles to be reported at a 1 h time resolution over a 20-day period. Internal mixing of silicate particles with nitrate, chlorine and organic–biological material was also measured and compared to that in the suspended soils. The results show SPMS and SEM techniques are complementary and demonstrate that SPMS can provide a meaningful high-resolution measurement of single-particle mineralogy and mixing state in laboratory and ambient conditions. In most cases, the differences in the mineralogical composition between particles within a soil sample were small. Thus, particles were not composed of discrete mineral phases. In ambient measurements, the ISCM and nitrate content was found to change significantly between distinct dust events, indicating a shift in source and transport pathways which may not be captured in offline composition analysis or remote sensing techniques.

Ice particle residuals (IRs) and the total aerosol particle population were sampled in parallel during mixedphase cloud events at the high-altitude research station Jungfraujoch in January-February 2017. Particles were sampled behind an ice-selective counterflow impactor (Ice-CVI) for IRs and a heated total inlet for the total aerosol particles. A dilution set-up was used to collect total particles with the same sampling duration as for IRs to prevent overloading of the substrates. About 4000 particles from 10 Ice-CVI samples (from 7 days of cloud events at temperatures at the site between −10 and −18 • C) were analysed and classified with operator-controlled scanning electron microscopy. Contamination particles (identified by their chemical composition), most likely originating from abrasion in the Ice-CVI and collection of secondary ice, were excluded from further analysis. Approximately 3000 total aerosol particles (IRs and interstitial particles) from 5 days in clouds were also analysed. Enrichment and depletion of the different particle groups (within the IR fraction relative to the total aerosol reservoir) are presented as an odds ratio relative to alumosilicate (particles only consisting of Al, Si, and O), which was chosen as reference due to the large enrichment of this group relative to total aerosol and the relatively high number concentration of this group in both total aerosol and the IR samples. Complex secondary particles and soot are the major particle groups in the total aerosol samples but are not found in the IR fraction and are hence strongly depleted. C-rich particles (most likely organic particles) showed a smaller enrichment compared to aluminosilicates by a factor of ∼ 20. The particle groups with enrichment similar to aluminosilicate are silica, Fe aluminosilicates, Ca-rich particles, Ca sulfates, sea-saltcontaining particles, and metal/metal oxide. Other aluminosilicates -consisting of variable amounts of Na, K, Ca, Si, Al, O, Ti, and Fe -are somewhat more enriched (factor ∼ 2) and Pb-rich particles are more (factor ∼ 8) enriched than aluminosilicates. None of the sampled IR groups showed a temperature or size dependence in respect to ice activity, which might be due to the limited sampling temperature interval and the similar size of the particles. Footprint plots and wind roses could explain the different total aerosol composition in one sample (carbonaceous particle emission from the urban/industrial area of Po Valley), but this did not affect the IR composition. Taking into account the relative abundance of the particle groups in total aerosol and the ice nucleation ability, we found that silica, aluminosilicates, and other aluminosilicates were the most important ice particle residuals at Jungfraujoch during the mixed-phase cloud events in winter 2017.

Objective: To assess changes in cardiovascular disease risk factors during a 3-year follow-up among 57 rotating shift workers and 29 day workers in industry. Methods: We collected demographics by questionnaire, examined blood pressure, heart rate, pulse wave velocity, carotid media thickness, and maximal oxygen uptake. We assessed blood samples for determination of lipids, glycosylated hemoglobin, C-reactive protein, markers of inflammation, and particle concentrations/respirable dust. Baseline comparisons were analyzed using logistic regression (plaque) and linear regression for all other outcomes. We applied mixed models to assess differences in change in health outcomes between the shift workers and the day workers. Results: At baseline, the adhesion molecules soluble vascular cell adhesion molecule 1 and soluble P-selectin were elevated among the shift workers compared with that of the day workers. There was a significant difference in change in pulse wave velocity between shift workers (1.29-m/s increase) and day workers (0.11-m/s increase) over the 3-year follow-up. Respirable dust levels were below the Norwegian occupational exposure limit. Conclusions: Shift work in industry is associated with arterial stiffening reflecting increased risk for future cardiovascular disease. More uncertainly, we found some support for systemic inflammation.

Abstract. The mineralogy and mixing state of dust particles originating from the African continent influences climate and marine ecosystems in the North Atlantic due to its effect on radiation, cloud properties and biogeochemical cycling. Single-particle mineralogy and mixing state is particularly important in many processes but is difficult to predict because of large temporal and spatial variability and the lack of in-situ measurements of dust properties during emission, transport and deposition. This lack of measurements is in part due to the remoteness of potential source areas (PSA) and transport pathways, but also because of 5 the lack of an efficient method to report the mineralogy and mixing state of single particles with a time resolution comparable to atmospheric processes.In this work, the mineralogy and mixing state of the fine fraction (< 2.5µm) in laboratory suspended dust from the Sahara and Sahel were made using novel techniques with on-line single-particle mass spectrometry (SPMS) and traditional off-line scanning electron microscopy (SEM). A regional difference in mineralogy was detected, with material sourced from Morocco 10 contained a high number fraction of illite like particles in contrast to Sahelian material which contains potassium and sodium depleted clay minerals like kaolinite. Applying the same methods to ambient measurement of transported dust in the marine boundary layer at Cabo Verde in the remote North Atlantic enabled the number fractions of illite/smectite clay mineral (ISCM), non-ISCM, and calcium containing particles to be reported at a 1 hour time resolution over a 20 day period alongside internal mixing with nitrate, sulphate and organic/biological material. The ISCM and nitrate content was found to change significantly 15 between distinct dust events, indicating a shift in source and transport pathways which may not be captured in off-line composition analysis or remote sensing techniques.The results show SPMS and SEM techniques are complimentary and demonstrate that SPMS can provide a meaningful high resolution measurement of single-particle mineralogy and mixing state in laboratory and ambient conditions. In most cases, the mineralogy varies continuously between particles rather than a collection of discrete mineral phases. These techniques 20 will be useful in resolving the complexity of mineral dust transport and in obtaining atmospherically relevant test material for laboratory experiments of dust properties.

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Mobilization of asbestos fibers from a 60-year-old corrugated asbestos cement roof was studied in material samples collected from a domestic house in Southern-Norway. Samples included pieces of the roof itself, rainfall simulated run off-water and moss growing on the roof surface. Weathered roof debris below the rain water system was investigated for asbestos fibers leached from the roof, and air samples were collected for asbestos concentrations in ambient air. Micrographs obtained by scanning electron microscopy show that weathering exposed asbestos fibers on the deteriorated roof surface. A high number of fibers found in the run off-water as well as in the weathered roof debris sample illustrates that exposed fibers are prone to mobilization. Investigation of weathering-induced magnesium depletion by scanning electron microscopy energy dispersive X-ray spectroscopy of asbestos fibers collected from the moss, roof underside and surface revealed that fibers had MgO weight % comparable to reference material. The major element chemistry of the fibers is therefore unaltered. Our findings demonstrate that care must be taken when working on and handling old corrugated asbestos cement roof as asbestos fibers might be released. In order to limit exposure to mobile fibers, moss covering asbestos cement roof should not be removed.