Phytochemistry, antioxidant and antibacterial activities of different solvent extracts of Chromolaena odorata leaves obtained by direct and sequential extraction were compared in this study. Antibacterial evaluation of the extracts was performed through a disc diffusion method. The results revealed that the solvents, Petroleum ether and Ethyl acetate showed the presence of a majority of the phytochemicals, Chloroform extract yielded maximum TPC and TAA and ethyl acetate yielded higher TFC when compared to other solvents. Ethanol and chloroform extracts showed lower concentrations of IC50 against DPPH and NO radicals respectively. Acetone recorded the maximum zone of inhibition against Staphylococcus aureus and Pseudomonas aeruginosa; ethyl acetate against Bacillus subtilis and Chloroform against Streptococcus mutans. Among the solvents studied for phytochemical content and bioactivities, ethanol showed a higher level of phytochemical extraction and better bioactivities. As a direct solvent extraction of C. odorata showed better efficiency when compared with sequential extraction, the study strongly recommends the use of a direct extraction method.
In the present study leaf tissue of host plant Calotropis procera was screened for the presence of endophytic fungi. The crude extract of the isolated endophytic fungi was tested for various chemical groups like alkaloids, flavonoids, diterpenoids and phenols. Our study showed the presence of 25 endophytic fungi isolated from the leaf tissue which constituted15 Hyphomycetes, 3 Ascomycetes, 3 Coelomycetes and 4 sterile forms. The qualitative analysis in all the 25 isolates showed the presence of alkaloid, phenol in 23 fungi, flavonoid in 19 and diterpenoids in 18 fungi. Fifteen endophytic fungi produced all chemical groups tested in the crude extract. Bipolaris sp a hyphomycete produced only one compound in culture. Among the four groups endophytic fungi Coelomycetes group able to produce all chemical groups tested.
Keywords: Endophytic fungi; Alkaloid; Flavonoid; Diterpenoids
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.