One of the problems of intensive cultivation of tilapia is the attack of Streptococcosis disease caused by Streptococcus agalactiae bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is Strychnine bush extract. The method used in this research is the experimental method using Completely Randomized Design (RAL), then the data is analyzed by using ANAVA. In this research, there were five treatments with various dosages, namely treatment (K: 0%), (A: 5%), (B: 10%), (C: 15%) and (D: 20%), each treatment was repeated four times. The main parameters used are hematocrit level, leukocrit level, leukocyte count and phagocytosis activity. As a supporting parameter is water quality and MIC (Minimum Inhibitation Concentration) test. The results showed that dosage of Strychnine bush extract did not affect the level of hematocrit and leukocrit level, but significant effect on leukocyte count with the highest value at treatment B with 10% dose is 11,9 × 105 cell / mm3. Giving extract also significant effect on phagocytosis activity with the highest phagocytosis value at treatment B with dose of 10% that is 32%.Keywords: Imune system, Streptococcosis, antibiotics, Minimum Inhibitation Concentration
One of the problemsofintensive catfish culture is the attack of Motile Aeromonas Septicemia disease caused by Aeromonas hydrophila bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is strychnine bush extract. The purpose of this research is to know the ability of strychnine bush extract (Strychnos ligustrina Blume) using methanol solvent in inhibiting the growth of Aeromonas hydrophila bacteria and its effect on prevalence and survival rate in Dumbo catfish (Clarias gariepinus). The method used in this research is the exsperimen method by using complete randomized design (RAL). In this study there were five treatments with different doses of treatment (P1: 6%), (P2: 12%), (P3: 18%), (P4: 24%) and (P5: 30%), each treatment was repeated three replications. Furthermore, the data were analyzed using ANAVA and continued with LSD test. The results showed that the giving of strychnine bush extract with different concentrations had a very significant effect on the width of the Aeromonas hydrophila bacterial resistance zone. The lowest inhibition of the treatment (P1: 6%) with a resistance of 1.79 mm. While the highest inhibition of treatment (P5: 30%) with 8.29 mm inhibition power. In prevalenceofdumbo catfish with givingofsongga extract very real effect, treatment with lowest prevalence is treatment (P4: 24%) with prevalence value 23,3% andhighestpervalence that is treatment (P5: 30%) with prevalence value equal to 40 %. In the synthesisofdumbo catfish with the giving of strychnine bush extract have a very real effect, the treatment with lowest stability is treatment (P4: 30%) with 77% syntax value and the highest synthesis of treatment (P5: 24%) with 90% syntax value. This study resulted in the conclusion that the best treatment for prevalence and survival rate is treatment (P4: 24%). As for the higher inhibition power dose strychnine bushextractis used then the higher the power inhibition.
Pertumbuhan dan kelulusan hidup benih ikan manfish (Pteropzhyllum scalare) yang dibudidayakan dengan kepadatan yang berbeda menggunakan sistem resirkulasi The growth and survival rate of manfish (Pterophyllum scalare) using different density in a recirculation aquaculture system PendahuluanIkan manfish Pterophyllum scalare dikenal sebagai ikan bidadari atau angelfish yang mempunyai kelebihan pada warna dan gerakannya yang anggun. Ikan ini terlihat cantik dengan gerakan yang lambat dan bentuk sirip yang berjumpai panjang membentuk ujung busur panah. Ikan manfish memiliki banyak strain yang masing-masing memiliki ciri khas tertentu, AbstrakPermintaan pasar yang tinggi pada ikan manfish sangat sulit terpenuhi dikarenakan pertumbuhan ikan manfish yang lambat. Solusi untuk memperbaiki pertumbuhan ikan manfish yaitu mengetahui padat tebar yang baik untuk ikan manfish serta ditambah penerapan teknologi resirkulasi yang di harapkan pertumbuhan ikan menjadi lebih cepat. Berdasarkan permasalahan itu maka dilakukannya penelitian ini untuk melihat respon ikan terhadap pertumbuhan. Metode yang digunakan dalam penelitian ini adalah metode Rancangan Acak Lengkap. Penelitian ini menggunakan 4 perlakuan (P1 (3 ekor/L dengan 25 liter air yaitu 75 ekor/wadah), P2 (6 ekor/L dengan 25 liter air yaitu 150 ekor/wadah), P3 (9 ekor/L dengan 25 liter air yaitu 225 ekor/wadah), dan P4 (12 ekor/L dengan 25 liter air yaitu 300 ekor/wadah) yang masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil penelitian menunjukkan bahwa nilai rerata SGR (specific growth rate) yang terbaik adalah pada pelakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata SGR sebesar 5,12 %. Laju pertumbuhan panjang yang terbaik adalah pada pelakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata laju pertumbuhan panjang sebesar 1,40 %. Kelangsungan hidup (survival rate) terbesar adalah pada perlakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata kelangsungan hidup (survival rate) sebesar 86,22%. Secara statistik pada penelitian ini mendapatkan hasil berpengaruh nyata terdapat pertumbuhan dan kelulusan hidup. AbstractHigh market demand for manfish is challenging to fulfill due to the slow growth of manfish. The solution to improve the growth of manfish is knowing good stocking density for manfish and adding the application of recirculation technology, which is expected to accelerate fish growth. Based on these problems, this study was conducted to see the response of fish to growth. The method used in this study is a completely randomized design method. In this study, we were using four treatments (P0 (3 fish/L with 25 liters of water is 75 fish/aquarium), P1 (6 fish/L with 25 liters of water is 150 fish/aquarium), P2 (9 fish /L with 25 liters water which is 225 fish/aquarium), and P3 (12 fish/L) with 25 liters of water, i.e., 300 fish/aquarium), each of which is repeated three times. The results show that the best SGR (specific growth rate) was in the tr...
Nile Tilapia or Tilapia Fish (Oreochromis niloticus) is one of commodity with a high number of consumers. But, Streptococcus agalactiae attack caused a decline in production. One way to reduce these diseases by using natural materials of songga wood (Strychnos lucida R.Br) where the songga wood contains many compounds like alkaloid, phenols, flavonoids, triterpenoids, which can inhibit bacterial growth by damaged cell membrane. The results showed that the inhibitory songga wood extract against Streptococcus agalactiae was highly significant, and shows with the highest survival rate of 90% and proved by theincreasingantibodytitersafteraddingsonggawood extract.
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