Background: Academic stress is a major problem that often occurs in nursing students. Islamic spiritual mindfulness (ISM) is an adaptive coping strategy used to mitigate various negative psychological reactions to respond stressors experienced by the body to build self-awareness that any problems experienced today are the scenario of Allah.Purpose: This study aimed to determine the effects of Islamic spiritual mindfulness therapy to reduce stress.Methods: This study used a pre-post quasi-experimental design with a control group. Participants were bachelor students of nursing who are Muslim and experienced stress in working on the thesis (n=36) and were assigned to two groups: the experimental group receiving Islamic spiritual mindfulness and the control group. Data were collected using questionnaires of Depression Anxiety Stress Scale (DASS) and analyzed using paired t-test and unpaired t-test.  Results: The results showed that the mean stress level of students in the intervention group decreased from 20.6±2.97 to 11.4±5.81 after the intervention. Meanwhile, in the control group, the mean student stress level slightly decreased from 19.7±2.82 to 17.8±5.01. The t-test obtained a p-value of 0.001, indicating that there were significant differences in stress levels between the intervention group and the control group. The effect size obtained the value of 0.59, meaning the level of influence was in the medium category.Conclusion: This study concluded that Islamic spiritual mindfulness was effective in reducing stress among nursing students working on the thesis. It is recommended for the nursing profession, especially mental health nurses, to apply Islamic spiritual mindfulness therapy as one of the psychotherapy interventions.

Coelomic fluid of Lumbricus rubellus (CFL) has attracted interest due to its pharmacological properties, including antitumor effect. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. This study aims to investigate whether the combination of CFL and 5-fluorouracil could reduce FAK protein level and iCa2+ and enhance p21 level. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. After 24 hours of treatment, it was necessary to assess the percentage of apoptosis, FAK, and p21 protein expression by flow cytometry. iCa2+ concentration was measured using immunofluorescence. The combination therapy of CFL with 5-fluorouracil potently suppressed six treatment groups were included in this study. HT-29 cell lines were cultured and divided into six groups: group 1 was treated with vehicle (negative control), groups 2-5 were treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5-fluorouracil, and group 6 was treated with CFL 20 µg/ml, the progression of colorectal cancer. Combination of CFL and 5-fluorouracil significantly decreased FAK expression (p<0.05), iCa2+ (p<0.05), and increased p21 expression (p<0.05) in HT-29 cells. Our results suggest that CFL has an anticancer potential in colorectal cancer when combined with 5-fluorouracil.

Objectives The aim of the study was to analyze the correlation between periodontitis severity in systemic lupus erythematosus (SLE) with CD4/CD8 lymphocytes ratio and cytomegalovirus gamma immunoglobulin (IgG CMV) level. Materials and Methods This is a descriptive study using a cross-sectional approach that included 93 subjects who were diagnosed with SLE in Rheumatology Department, Saiful Anwar Hospital, during 2017 to 2019. Periodontitis severity was assessed by periodontal Index (PI). CD4/CD8 lymphocyte ratio was determined using flow cytometry and IgG CMV levels using enzyme-linked immunosorbent assay. Statistical Analysis The differences among the three groups were analyzed using analysis of variance. Correlation among the groups was calculated using Spearman/Pearson correlation coefficient test, while regression analysis was done using Statistical Package for the Social Sciences. Results The mean of periodontitis severity and standard deviation in SLE was 2.66 ± 1.02. There were negative correlation between CD4/CD8 lymphocyte ratio with periodontal index (r = –0.971) and positive correlation between IgG CMV level with periodontal index (r = 0.977). Conclusions Inverted CD4/CD8 ratio and IgG CMV were found associated with periodontitis severity in SLE patient. Further research was recomended that CD4/CD8 lymphocytes ratio and IgG CMV can be used as a potensial marker of periodontitis severity in SLE patients

No abstract

Background Vitiligo is a progressive depigmentation of the skin with unclear etiology. Cell-mediated immunity has been suggested to play an important role in the pathogenesis of vitiligo’s progression. Melan-A has a high affinity for specific CD8+ T cells and is one of the critical markers for detecting damage to melanocytes. Our study aims to demonstrate the differences in Melan-A expression associated with apoptosis of melanocytes in patients with segmental vitiligo (SV) and those with non-segmental vitiligo (NSV). Methods A cross-sectional study with 64 patients diagnosed with vitiligo, of whom 33 had NSV and 31 had SV. Skin biopsy and direct immunofluorescence were used to examine Melan-A, and the TUNEL staining method was performed to examine melanocyte apoptosis in both groups. Group comparisons were conducted using appropriate statistical methods. Results Melan-A expression was significantly higher in the NSV group than in the SV group, and there was a significant difference between the two groups (p=0.001). The median of melanocyte apoptosis in the NSV group was relatively higher than in the SV group, and a significant difference was found between the two groups (p=0.001). The Spearman’s rank correlation test between Melan-A expression and melanocyte apoptosis in the NSV group was 0.767 (76.7%) and showed a significant relationship (p<0.05). The same test in the SV group was 0.583 (58.3%) and showed a significant relationship (p<0.05). In both groups, the higher the Melan-A expression, the higher the melanocyte apoptosis. Conclusion Melan-A expression and melanocyte apoptosis are positively correlated. Higher Melan-A expression and melanocyte apoptosis in NSV indicates more severe vitiligo disease compared to SV.

Aims and Scope Eurasian Journal of Medicine (Eurasian J Med) is an international, scientific, open access periodical published by independent, unbiased, and tripleblinded peer-review principles. The journal is the official publication of

Sindrom Koroner Akut (SKA) merupakan kondisi kegawatan yang paling sering mengakibatkan kematian. Penyakit ini membutuhkan penatalaksaan yang cepat dan tepat, tetapi yang sering terjadi adalah waktu keterlambatan prehospital yang panjang. Penyebab keterlambatan prehospital dikaitkan dengan persepsi pasien tentang nyeri kardiak. Tujuan penelitian yaitu untuk menjelaskan hubungan persepsi nyeri kardiak dengan keterlambatan prehospital pada pasien SKA di IGD RSUD dr. T.C. Hillers Maumere. Jenis penelitian observasional analitik dengan pendekatan cross sectional. Sampling yang digunakan adalah consecutive sampling dengan besar sampel sebanyak 42 orang. Data dikumpulkan dengan lembar wawancara pada bulan April-Juni 2017, kemudian di analisis secara univariat dan bivariat (uji contingency coefficient). Hasil penelitian menunjukkan sebanyak 28 pasien (66.7%) mempersepsikan nyeri kardiak adalah bukan penyakit jantung, pasien tiba terlambat di IGD sebanyak 26 pasien (61.9%). Hasil uji contingency coefficient diperoleh ada hubungan persepsi nyeri kardiak dengan keterlambatan prehospital pada pasien SKA di IGD RSUD dr. T.C. Hillers Maumere (p=0.002). Nilai contingency coefficient sebesar 0.437, maka dapat disimpulkan hubungan ke dua variabel dalam kategori sedang. Penelitian ini mengindikasikan pentingnya penyuluhan kesehatan untuk meningkatkan persepsi pasien yang sesuai.

The use of insect repellent is a disease preventive effort due to mosquito vectors as well as sources of pollutants that disrupt human health, especially reproductive health. This study aimed to determine the effect of γ-Oryzanol on the expression of Foxo3a, TNF-α, GDF-9 in the folliculogenesis of mice (Rattus Novergicus) exposed to pyrethroid aerosol mosquitoes. The research use bioinformatics technique with in silico approach. The result of study showing that γ-oryzanol has the potential as an anti-inflammatory and NO scavenger, the influence of the active compound γ-oryzanol on Foxo3 expression obtained pathway analysis with the results of the prediction of the target HITPICK able to bind to APP, AKT1 and Mtor expressions through modification of post-translational Foxo1, Effect of γ-oryzanol on Gdf9 expression, through bonding with APP, AKT1, AKT3, Pik3cg and modifying Smad 4 post-translation, The effect of the active compound γ-oryzanol on TNF-α expression via APP binds with AKT1, AKT3, AKT2 then activates IkbkB and bindings with Casp3, Fadd, Tradd, Tnfrsf1a, Tnfrsf1b, Traf3, Birc2, Ikbkg. Based on average part length analysis AKT1 is the fastest interact with protein target. The conclusion, γ-oryzanol has predicted to be used for the treatment of insecticide exposure, because it can act as an anti-inflamatory, and lipid perosidase inhibitor. This condition influences normally folliculogenesis with stable expression of Foxo3a, GDF 9 and TNF-α.