Deep-brain microscopy is strongly limited by the size of the imaging probe, both in terms of achievable resolution and potential trauma due to surgery. Here, we show that a segment of an ultra-thin multi-mode fiber (cannula) can replace the bulky microscope objective inside the brain. By creating a self-consistent deep neural network that is trained to reconstruct anthropocentric images from the raw signal transported by the cannula, we demonstrate a single-cell resolution (< 10μm), depth sectioning resolution of 40 μm, and field of view of 200 μm, all with green-fluorescent-protein labelled neurons imaged at depths as large as 1.4 mm from the brain surface. Since ground-truth images at these depths are challenging to obtain in vivo, we propose a novel ensemble method that averages the reconstructed images from disparate deep-neural-network architectures. Finally, we demonstrate dynamic imaging of moving GCaMp-labelled C. elegans worms. Our approach dramatically simplifies deep-brain microscopy.
A solid-glass cannula serves as a micro-endoscope that can deliver excitation light deep inside tissue while also collecting emitted fluorescence. Then, we utilize deep neural networks to reconstruct images from the collected intensity distributions. By using a commercially available dual-cannula probe, and training a separate deep neural network for each cannula, we effectively double the field of view compared to prior work. We demonstrated ex vivo imaging of fluorescent beads and brain slices and in vivo imaging from whole brains. We clearly resolved 4 µm beads, with FOV from each cannula of 0.2 mm (diameter), and produced images from a depth of ∼1.2 mm in the whole brain, currently limited primarily by the labeling. Since no scanning is required, fast widefield fluorescence imaging limited primarily by the brightness of the fluorophores, collection efficiency of our system, and the frame rate of the camera becomes possible.
Many deep learning approaches to solve computational imaging problems have proven successful through relying solely on the data. However, when applied to the raw output of a bare (optics-free) image sensor, these methods fail to reconstruct target images that are structurally diverse. In this work we propose a self-consistent supervised model that learns not only the inverse, but also the forward model to better constrain the predictions through encouraging the network to model the ideal bijective imaging system. To do this, we employ cycle consistency alongside traditional reconstruction losses, both of which we show are needed for incoherent optics-free image reconstruction. By eliminating all optics, we demonstrate imaging with the thinnest camera possible.
We demonstrate optics-free imaging of complex color and monochrome QR-codes using a bare image sensor and trained artificial neural networks (ANNs). The ANN is trained to interpret the raw sensor data for human visualization. The image sensor is placed at a specified gap (1mm, 5mm and 10mm) from the QR code. We studied the robustness of our approach by experimentally testing the output of the ANNs with system perturbations of this gap, and the translational and rotational alignments of the QR code to the image sensor. Our demonstration opens us the possibility of using completely optics-free, non-anthropocentric cameras for application-specific imaging of complex, non-sparse objects.
Purpose The purpose of this paper is to review the techniques for versatile advancements in contact tracing for the coronavirus disease 2019 (COVID-19) positive cases in this pandemic and to introduce the way of using the mobile location information collected within the country India. As the method, an exploratory review of current measures was conducted for confirmed COVID-19 contact tracing after understanding the current situation of the world. This paper has examined the way of using free locational information in an innovative way to reduce the spread of COVID-19 spread. Design/methodology/approach COVID-19 pandemic is the utmost global economic and health challenge of the century. One powerful and consistent procedure to slow down the spread and decrease the effect of COVID-19 is to track the essential and auxiliary contacts of confirmed COVID-19 positive cases by using contact-tracing innovation. Findings Although it takes the information from various clients, there are numerous odds in the information. The sincere measures were taken by the authors to avoid the abuse of information by any kind. A portion of the tips for keeping information from getting abused is on the whole, the information ought to be with just higher specialists, and they ought not to have the authorization to impart information to anybody. Originality/value This paper helps to track the COVID-19 positive cases as of now by using the field information assortment and outbreak examination stages. At the same time, mobile location information used inside the current guideline, rules for information handlers must incorporate measures to reduce the abusing of information.
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