The interaction between Saccharomyces cerevisiae and chrysotile fibers was studied by scanning electron microscopy. The yeast cells adhere preferentially to the fibrils. In the extreme case, all the adhered fibrils were broken, resulting in a complete coverage of the surface. The chrysotile covered cells showed less buds, but retained metabolic capacities, and were fully active in fermentation experiments after one year. The interaction degree was depending on contact time and adhesion medium. The longer the contact period, the stronger the interaction between the cells and the fibers. Cells adhered in water show poor entrapment after short contact time, but were highly entrapped after longer periods and did not show any agglomerates. Cells adhered in the presence of nutrients showed a lower entrapment and a higher degree of cellular growth.
Estudamos o comportamento dos parâmetros fenomenológicos de intensidade das transições 4f-4f em compostos de Nd 3+ com glicina, ácido L-aspártico, ácido L-glutâmico, L-histidina, ácido DL-málico e Aspartame® em solução aquosa como função dos valores de pK e das cargas parciais sobre os átomos de oxigênio dos grupos carboxilatos dessas moléculas. Os resultados são discutidos e interpretados qualitativamente em termos dos mecanismos das intensidades 4f-4f por dipolo elétrico forçado e acoplamento dinâmico, indicando como dominante o mecanismo de dipolo elétrico forçado.We have studied the bevahior of the phenomenological 4f-4f intensity parameters in compounds of the Nd 3+ ion with glycine, L-aspartic acid, L-glutamic acid, L-histidine, DL-malic acid and Aspartame TM in aqueous solution, as a function of the pK values and partial charges on the oxygens of the carboxylate groups of these molecules. The results are discussed and qualitatively interpreted in terms of the forced electric dipole and dynamic coupling mechanisms of the 4f-4f intensities, thus indicating that the forced electric dipole mechanism is dominant.
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