Nitric oxide radical scavenging activityNOSA was performed as per previous method of Kumar et al.,11 10μg, 50μg and 100μg concentrations of aqueous and methanolic plant extract and Butylated hydroxy anisole (BHA) were taken in different test tubes and made up to 3ml with 0.1M phosphate buffer J Anal Pharm Res. 2019;8(3):94-97. 94 AbstractPresent study was undertaken to investigate phytochemical, nitric oxide scavenging activity and antihypertensive activity of the methanolic and aqueous leaf extracts of S. oleosa. The phytochemical analysis carried out revealed the presence of flavanoids, glycosides, alkaloids, tannins, saponins and steroids and many other metabolites. Both the extracts showed high NOSA (33.81% and 31.43% NOSA at 100µg concentration). The methanolic leaf extract showed 1.6%, 4%, and 5.6% ACE inhibition against 10µg, 50µg and 100µg concentration respectively and aqueous leaf extract of S. oleosa showed very negligible percentage that is 0%, 0% and 0.8% ACE inhibition against 10µg, 50µg and 100µg extract concentration respectively while Captopril showed 100% ACE inhibition against all the three concentrations. The result shows that the methanolic as well as the aqueous leaf extracts of Schleichera oleosa are not very good antihypertensive agents. However the methanolic leaf extract of Schleichera oleosa is a better antihypertensive inhibitor than the aqueous leaf extract.
In this study a comparative analysis of total anti-oxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa was done by estimation of DPPH radical, hydroxyl radical, superoxide anion radical and nitric oxide radical scavenging activity. The methanolic extract (M.E) of S.oleosa showed a maximum concentration of total phenolics and flavanoids up to 20% and 168.89 % and aqueous extract (A.E) showed a maximum concentration of total phenolics and flavanoids up to 4.90% and 9.33% respectively. The M.E of S.dulcis showed a maximum concentration of total phenolics and flavanoids up to 6.21% and 9.24 % and A.E 46.22% and 14.67% respectively. Free radical scavenging activity was observed in both the M.E and A.E. For S.dulcis the % of free radical activity in M.E and A.E in 100µg concentration was 2.98% and 15.53% respectively. M.E and A.E of S.oleosa showed 54.94% and 5.93 % free radical scavenging activity correspondingly. Hydroxyl scavenging activity for 100µg of S.dulcis extracts were 17.92% in M.E and 11.39% in A.E where as for S.oleosa extracts it was 35.83% and 8.19% in M.E and A.E respectively. S.dulcis showed only 4.03% and 3.23% scavenging of superoxide anion radical and S.oleosa showed superoxide anion scavenging up to 22.58% in M.E and 3.23% in A.E. Nitric oxide scavenging activity was 5.71% in M.E of S.dulcis and 5.24% for A.E where as S. oleosa showed 33.81% nitric oxide scavenging activity in M.E and 31.43 % in A.E. The presence of phenol, flavonoid and total antioxidant in both the extract justifies the antioxidant potential of the plants which brings about their free radicals scavenging potential. Thus we conclude that the antioxidant activities may be due to the cumulative effect of the phytochemicals present in the plant which genuinely designate them as free radical scavengers.
Background: The present study aims to study the Hormonal profile and haematological parameters of Wistar albino rats treated with methanolic and aqueous extracts of Schleichera oleosa.Result: The amount of RBC was found to be 9.6±0.057106/mm3, 9.7±0.0125, 106/ 9.5±0.003, 106/mm3 9.5±0.012 106/mm3 and 9.5±0.023 106/mm3. The amount of WBC was found to be 13.5±0.058 103/mm3,13.6±0.0113 103/mm3,13.7±0.021 103/mm3, 9.5±0.012 103/mm3 and 9.5±0.023 103/mm3. Platelets, 396±0.577 103/mm3, 395±0.03 103/mm3 , 397±0.042103/mm3 , 398±0.020103/mm3, and 397±0.011103/mm3 for controlled group, low dose of methanolic extract, high dose of methanolic extract, low dose of aqueous extract and high dose of aqueous extract respectively. The packed cell volume, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin, corpuscular haemoglobin concentration and amount of neutrophils, Eosinophils, Lymphocytes, Basophils and Monocytes were measured and no significant change was noticed. The amount of testosterone showed significant increase from 0.75± 0.024 pg/ mL (control) 2.96± 0.088 pg/ mL, 3.9± 0.14pg / mL, 1.85± 0.076 pg/ mL and 2.85± 0.074 pg/ mL.Conclusions: The study suggests that the prolonged treatment of Wistar rats with both low dose and high dose of methanolic as well as the aqueous extract of Schleichera oleosa is not toxic and they increased the amount of testosterone in the blood which is indicative of their testosterone enhancement.
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