BackgroundHematology analyzers may ineffectively recognize abnormal cells, and manual differential counts may be imprecise for leukopenic samples. We evaluated the efficacy of the Hematoflow method for determining the leukocyte differential in leukopenic samples and compared this method with the manual differential method.MethodsWe selected 249 blood samples from 167 patients with leukopenia (WBC counts, 500-2,000/µL) for analysis in this study. The EDTA-anticoagulated blood samples were analyzed using an automatic blood cell counter (DxH800; Beckman Coulter, USA) and flow cytometry (FC 500; Beckman Coulter) by using Cytodiff reagent and analysis software (Beckman Coulter). Hematoflow results were selected or calculated from DxH800 and Cytodiff results. Two trained pathologists performed a manual differential count by counting 50-100 cells.ResultsThe precision of the Hematoflow method was superior to that of the manual method in counting 5 leukocyte subpopulations, immature granulocytes (IGs), and blasts. Blasts were detected in all 45 cases (100%) by Hematoflow. The correlation of the Cytodiff blast count to the reference count was high (r = 0.8325). For all other cell populations, the correlation of the Hematoflow results with the reference count was stronger than that of the other manual counts with the reference count.ConclusionsThe Hematoflow differential counting method is more reproducible and sensitive than manual counting, and is relatively easy to perform. In particular, this method detected leukemic blasts more sensitively than manual differential counts. The Hematoflow method is a very useful supplement to automated cell counting.
The purpose of this study was to investigate the hydrophilicity of dental impression materials. Two ultrahydrophilic (Fusion, Genie) and four hydrophilic (Imprint II, Aquasilultra, Twinz, Perfect-F) impression materials were used. Initially, 0.5% NaOCl and 1% povidone were used as disinfectants. In order to investigate the change of the surface wettability of impression materials according to the elapsed time after mixing, the contact angles were measured at 30 s and 2 min after mixing, respectively. Contact angles were also measured before and after disinfection. The contact angles of the ultrahydrophilic impression materials were lower at 2 min than at 30 s, whereas those of the hydrophilic impression materials showed the opposite result (p < 0.05). Fusion and Perfect-F had higher contact angles in the NaOCl-group and lower contact angles in the povidone-group than those of nondisinfection group (p < 0.05). The contact angles of Aquasilultra and Twinz in the povidone-group were higher than those in the nondisinfection group (p < 0.05). The elapsed time after mixing and disinfection of impression materials affected the contact angle of water on the surface of the impression materials. These results suggested that impression materials should only be used within the scope that their wettability is not degraded.
This study involves enhancing the performance of the Na(Li,Ti)O 2 system as an Na-ion battery anode with the addition of Mg, which partially replaces Li ions. We perform both computational and experimental approaches to achieve a higher reversible capacity and a faster transport of Na ions for the devised system. Computational results indicate that the Na(Li,Mg,Ti)O 2 system can provide a lower-barrier path for Na-ion diffusion than can a system without the addition of Mg. Experimentally, we synthesize various Na z (Li y ,Mg x ,Ti)O 2 systems and evaluate their electrochemical characteristics. In agreement with the theoretical study, Mg addition to such systems improves general cell performance. For example, the prepared Na 0.646 (Li 0.207 Mg 0.013 Ti 0.78)O 2 system displays an increase in reversible capacity of 8.5% and in rate performance of 13.5%, compared to those characteristics of a system without the addition of Mg. Computational results indicate that these improvements can be attributed to the slight widening of the Na-O 6 layer in the presence of Mg in the (Li,Ti)O 6 layer.
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