Spinal
cord injury (SCI) generally leads to long-term functional
deficits and is difficult to repair spontaneously. Many biological
scaffold materials and stem cell treatment strategies have been explored,
but very little research focused on the method of combining exogenous
neural stem cells (NSCs) with a biodegradable conductive hydrogel
scaffold. Here, a NSC loaded conductive hydrogel scaffold (named ICH/NSCs)
was assembled by amino-modified gelatin (NH2-Gelatin) and
aniline tetramer grafted oxidized hyaluronic acid (AT-OHA). Desirably,
the well-conducting ICH/NSCs can be simply injected into the target
site of SCI for establishing a good electrical signal pathway of cells,
and the proper degradation cycle facilitates new nerve growth. In vitro experiments indicated that the inherent electroactive
microenvironment of the hydrogel could better manipulate the differentiation
of NSCs into neurons and inhibit the formation of glial cells and
scars. Collectively, the ICH/NSC scaffold has successfully stimulated
the recovery of SCI and may provide a promising treatment strategy
for SCI repair.
The treatment and management of diabetic foot ulcers (DFUs) is a pretty intractable problem for clinical nursing. Urgently, the “Black Box” status of the healing process prevents surgeons from providing...
BackgroundA growing body of evidence supports the involvement of long noncoding RNA 00152 (LINC00152) in the progression and metastasis of multiple cancers. However, the exact roles of LINC00152 in the progression of human retinoblastoma (RB) remain unknown. We explored the expression and biological function of human RB.Materials and methodsThe expression level of LINC00152 in RB tissues and cells was analyzed using quantitative real-time PCR. The function of LINC00152 was determined using a series of in vitro assays. In vivo, a nude mouse model was established to analyze the function of LINC00152. Gene and protein expressions were detected using quantitative real-time PCR and Western blot assays, respectively.ResultsThe expression of LINC00152 mRNA was upregulated in RB tissues and cell lines. Knockdown of LINC00152 significantly inhibited cell proliferation, colony formation, migration, and invasion and promoted cell apoptosis and caspase-3 and caspase-8 activities in vitro, as well as suppressing tumorigenesis in vivo. We identified several genes related to proliferation, apoptosis, and invasion including Ki-67, Bcl-2, and MMP-9 that were transcriptionally inactivated by LINC00152.ConclusionTaken together, these data implicate LINC00152 as a therapeutic target in RB.
A modified structure
Ca(Mg0.8Al0.2)(Si1.8Al0.2)O6 (denoted as CMASO) from the evolution of CaMgSi2O6 (denoted as CMSO) codoped with Ce3+ and Tb3+ ions was designed successfully by solid reaction
method for application in phosphor-converted white-light-emitting
diode (pc-wLED). The Rietveld refinement of these two structures verified
the changes derived from the replacement of some of the Mg2+ and Si4+ ions by Al3+ ions. The band gaps
were calculated by density-functional theory (DFT) calculation method
to verify the change of Al3+ ions replacing further, and
the diffuse reflectance spectra (DRS) proved the veracity of the calculation
result. The phosphors
CMASO:Ce3+ showed blue emission excited by a wider excitation
wavelength from 280 nm to 370 nm. The change of structure lead to
the absorbable range broaden and the emission peak shifted to longer
wavelength, compared with CMSO:Ce3+, although the amount
of emitting center was the same. The reason for these phenomena was
discussed in detail. The codoped phosphors CMASO:Ce3+,Tb3+ exhibited different emission colors from blue to green as
the concentration of Tb3+ ions increased. Combined with
commercial red phosphor CaAlSiN3:Eu2+ and ultraviolet
LED (UV-LED) chips, the selected appropriate samples achieved white
emission. The correlated color temperature (CCT) was 6137 K and the
color rendering index (Ra) was 80.5, indicating that they could act
as potential phosphors for possible applications in pc-wLED.
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