Knockdown of long noncoding RNA 00152 (LINC00152) inhibits human retinoblastoma progression

Li, Songhe; Wen, Dacheng; Che, Songtian; Cui, Zhi-Hua; Sun, Yabin; Ren, Hua; Hao, Ji‐Long

doi:10.2147/ott.s160428

Cited by 18 publications

(12 citation statements)

References 27 publications

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“…Accumulating literatures have highlighted that the expression change of lncRNAs has critical and clinically predictive roles in tumorigenesis (Elchuri et al, 2018). More and more lncRNAs were repor- (Hu et al, 2018), MT1JP (Bi et al, 2018), LIN00152 (Li et al, 2018), CCAT1 (Zhang et al, 2017), BANCR (Su et al, 2015), H19 (Zhang et al, 2018a), BDNF-AS (Shang et al, 2018), MALAT1 (Liu et al, 2017) and HOTAIR (Dong et al, 2016). Except for MT1JP and BDNF-AS, which are down-regulated and act as a tumor suppressor gene in RB, other lncRNAs are all up-regulated in RB and promote RB progression.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma

Yan

et al. 2019

Cell Struct. Funct.

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Retinoblastoma (RB) is the most common intraocular malignancy in childhood, and the prognosis in the advanced RB is poor. It is urgent to find novel therapeutic targets. Long noncoding RNAs (lncRNAs) have critical functions in cancer progression, and lncRNA LINC00202 is found associated with poor prognosis in RB. However, the functions of LINC00202 in RB remain unclear. We employed qRT-PCR and immunoblot to detect the expression levels of mRNAs and proteins, respectively. Cell proliferation was determined by CCK-8 assay and colony formation assay. Transwell assays were applied to evaluate the cell abilities of migration and invasion. Luciferase reporter assay was applied to examine RNA stability, and RNA pulldown assays were used to detect interaction between lncRNA and microRNA (miRNA). LINC00202 expression in RB tissues is higher than that in the paired adjacent normal tissues, which has correlation with poor prognosis in RB. RB cell proliferation, migration and invasion were weakened by LINC00202 depletion, but enhanced by LINC00202 overexpression. MiR-3619-5p was identified to directly bind and mediate LINC00202-promoted RB progression, meanwhile, miR-3619-5p directly regulated expression of an oncongene, RIN1. Moreover, RIN1 knockdown completely blocked miR-3619-5p-enhanced RB progression. In summary, high LINC00202 levels are correlated with poor prognosis in RB, and it promotes RB progression by sponging miR-3619-5p and therefore up-regulating RIN1 expression.

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Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma

Yan

et al. 2019

Cell Struct. Funct.

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“…For example, Hu et al reported that XIST promoted RB progression partially by modulating the miR-124/STAT3 axis 19. Li et al demonstrated that knockdown of LINC00152 significantly inhibited RB cell growth in vitro and in vivo 20. Zhang et al found that H19 inhibited RB progression via counteracting the roles of miR-17–92 cluster 21.…”

Section: Discussionmentioning

confidence: 99%

<p>Long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) promotes retinoblastoma progression via sponging miR-506-3p</p>

Han¹,

Zuo²,

Han³

et al. 2019

OTT

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Background: Long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) has been reported to be involved in initiation and development of multiple cancers. However, the detailed biological roles and underlying molecular mechanism of HOXA11-AS remain unclear in retinoblastoma (RB). Herein, the goals of this study were to explore the biological function and the potential mechanism of HOXA11-AS in RB. Materials and methods: The expression of HOXA11-AS in RB tissues and cell lines was detected using real-time PCR (qRT-PCR). Cell proliferation, cycle arrest and apoptosis were measured using a cell counting kit 8 and flow cytometry. The target miRNAs of HOXA11-AS was predicted by Starbase2.0 software and was confirmed using a dual-luciferase reporter assay. Result: We found that HOXA11-AS expression was markedly elevated in RB tissues and cell lines compared to normal retina tissues and human retinal epithelial cells, respectively. Functional analysis showed that knockdown of HOXA11-AS in RB cells significantly suppressed cell proliferation, and induced cell cycle arrest at G1/G0 phase and promoted cell apoptosis. We also found that HOXA11-AS could serve as a competing endogenous RNA (ceRNA) that inhibited miR-506-3p expression, which regulated its downstream target NIMA-related kinase 6 (NEK6) in RB. In addition, miR-506-3p inhibitors partially reversed the effect of HOXA11-AS depletion on proliferation, cycle arrest and apoptosis in RB cells. Conclusion: Taken together, these findings demonstrated that HOXA11-AS could promote RB progression by sponging miR-506-3p, suggesting that HOXA-11-AS might be a potential therapeutic target for RB.

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“…PlncRNA-1 (prostate cancer-up-regulated long noncoding RNA 1) modulates carbonyl reductase 3 (CBR3) activity, and LINC00152 (long noncoding RNA00152) inactivates Ki-67, Bcl-2, and MMP-9 at the post-transcriptional level. In both cases increased proliferation, invasion and migration were observed (Li et al, 2018b;Wang et al, 2018c).…”

Section: Ocular Malignancymentioning

confidence: 88%

Circular and long non-coding RNAs and their role in ophthalmologic diseases

et al. 2018

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Long non-coding RNAs are >200-nucleotide-long RNA molecules which lack or have limited protein-coding potential. They can regulate protein formation through several different mechanisms. Similarly, circular RNAs are reported to play a critical role in post-transcriptional gene regulation. Changes in the expression pattern of these molecules are known to underlie various diseases, including cancer, cardiovascular, neurological and immunological disorders (Rinn & Chang, 2012; Sun & Kraus, 2015). Recent studies suggest that they are differentially expressed both in healthy ocular tissues as well as in eye pathologies, such as neovascularization, proliferative vitreoretinopathy, glaucoma, cataract, ocular malignancy or even strabismus (Li et al., 2016). Aetiology of ocular diseases is multifactorial and combines genetic and environmental factors, including epigenetic and non-coding RNAs. In addition, disorders like diabetic retinopathy or age-related macular degeneration lack biomarkers for early detection as well as effective treatment methods that would allow controlling the disease progression at its early stages. The newly discovered non-coding RNAs seem to be the ideal candidates for novel molecular markers and therapeutic strategies. In this review, we summarized the current knowledge about gene expression regulators – long non-coding and circular RNA molecules in eye diseases.

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Knockdown of long noncoding RNA 00152 (LINC00152) inhibits human retinoblastoma progression

Cited by 18 publications

References 27 publications

Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma

Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma

<p>Long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) promotes retinoblastoma progression via sponging miR-506-3p</p>

Circular and long non-coding RNAs and their role in ophthalmologic diseases

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