ContentThe Golgi apparatus (GA) and endoplasmic reticulum (ER) play a central role in the events related to intracellular trafficking distribution. This work evaluated the dynamics and localization of the GA and ER in canine oocytes during meiotic development in vitro. Cumulus-oocytes complexes (COCs) from ovaries of adult bitches were incubated for IVM for 0, 48, 72 and 96 h. At each time, the nuclear status was determined using DAPI staining, and the GA was evaluated by immunofluorescence using two antibodies against Golgi proteins: GM130 and Giantin. ER was analysed with fluorescent lipid probes (ER-Tracker), for living cells. Golgi structures were homogeneous in the cytoplasm in non-matured oocytes, mainly in those GV-arrested oocytes. In contrast, at 48 h and from GVBD stage, the immunolocalization began to be subcortical, increasing at 72 h and 96 h. Meiotic development increased with time and the majority of oocytes at MI-MII stages showed cortical distribution of Golgi structure. Living ZP intact non-matured oocytes showed a reticular pattern of ER that covered oocyte cortex. Confocal microscopy showed that, in all levels cuts the fluorescence marks were located in the cortical region, irrespective of culture time. The changes and localization in these organelles during IVM might be related to meiotic development, but in a non-synchronous manner.
ENSAYO RESUMENEn las últimas dos décadas, Chile ha incrementado su tasa de inmigración nacional: la última estimación determinó que, para el año 2014, el 2,3 % de la población nacional correspondería a migrantes permanentes, lo cual se ha duplicado en los últimos 12 años. Ante este panorama, es necesario que la formación de profesionales de enfermería se adapte con el fin de responder a las nuevas necesidades sociales del país y, consecuentemente, brindar cuidados considerando la diversidad cultural. El siguiente texto invita a reflexionar sobre la preparación brindada a los enfermeros en formación para la entrega de cuidados culturales sensibles, mediante la adquisición de competencias culturales. A partir de las evidencias, incluir el desarrollo de las competencias culturales en los diversos planes de estudio no es tarea fácil, por el contrario, es un desafío inmensurable. En el contexto de la innovación curricular de la carrera de Enfermería de la Universidad de Chile, se están implementando acciones orientadas a formar competencias culturales a través de la inclusión de un curso: de acuerdo con docentes y estudiantes, la experiencia es satisfactoria y necesaria, pero insuficiente para lograr el objetivo esperado. Dado lo anterior, es indispensable que los profesionales de enfermería desarrollen competencias culturales, máxime ante la oportunidad de generar nuevas estrategias que contribuyan al desarrollo de estas y de generar un modelo que permita avanzar en una gestión del cuidado más equitativa, inclusiva y con pertinencia cultural.Palabras clave: Educación-en-Enfermería, Educación-Superior, Enfermería-Transcultural. ABSTRACTIn the last two decades Chile has increased its national immigration rate. The latest estimate determined that by the year 2014 2.3% of the national population will correspond to permanent migrants, doubling in the last 12 years. Given this scenario it is necessary that the training of nursing professionals is adapted in order to respond to the new social needs of the country and consequently to be able to provide care considering cultural diversity.The text invites to reflect on the preparation that is given to nurses in training for the delivery of sensitive cultural care, through the acquisition of cultural skills. In the light of the evidence it is no easy task, including the development of cultural competitions in the various curricula it is an immeasurable challenge. In the context of the curricular innovation of the Nursing career of the University of Chile, actions are being carried out aimed at the formation of cultural competitions through the implementation of a course. According to the perception of teachers and students the experience is satisfactory and necessary but still insufficient to achieve the expected objective. It is undoubtedly essential that nurses develop cultural competencies. As teachers we have the opportunity to generate new strategies that contribute to the development of these and to create a model that allows progress in a management of care that is mo...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.