Golgi Apparatus and Endoplasmic Reticulum Dynamic during Meiotic Development in Canine Oocytes

Abstract

ContentThe Golgi apparatus (GA) and endoplasmic reticulum (ER) play a central role in the events related to intracellular trafficking distribution. This work evaluated the dynamics and localization of the GA and ER in canine oocytes during meiotic development in vitro. Cumulus-oocytes complexes (COCs) from ovaries of adult bitches were incubated for IVM for 0, 48, 72 and 96 h. At each time, the nuclear status was determined using DAPI staining, and the GA was evaluated by immunofluorescence using two antibodies against Golgi proteins: GM130 and Giantin. ER was analysed with fluorescent lipid probes (ER-Tracker), for living cells. Golgi structures were homogeneous in the cytoplasm in non-matured oocytes, mainly in those GV-arrested oocytes. In contrast, at 48 h and from GVBD stage, the immunolocalization began to be subcortical, increasing at 72 h and 96 h. Meiotic development increased with time and the majority of oocytes at MI-MII stages showed cortical distribution of Golgi structure. Living ZP intact non-matured oocytes showed a reticular pattern of ER that covered oocyte cortex. Confocal microscopy showed that, in all levels cuts the fluorescence marks were located in the cortical region, irrespective of culture time. The changes and localization in these organelles during IVM might be related to meiotic development, but in a non-synchronous manner.

High Temperature Disrupts Organelle Distribution and Functions Affecting Meiotic Maturation in Porcine Oocytes

“…Therefore, based on the results of ER distribution and function, we investigated the effect of HT exposure on distribution and function of the Golgi apparatus. Normally, the Golgi apparatus is fragmented and dispersed in the cortical area in canine MII oocytes (De los Reyes et al, 2012). The number of Golgi apparatus membranous vesicles in the cortex increases following oocyte maturation in mouse oocytes (Ducibella et al, 1988).…”

“…Following oocyte maturation, ER is redistributed and localized in the cortex, and forms clusters, which are usually 1-2 mm in diameter (Kline, 2000). Localization of ER clusters is analogous to the distribution of polarized CGs (Kline, 2000), and is essential for preparing oocytes for the generation of rapid calcium waves, which induce oocyte activation during fertilization (De los Reyes et al, 2012). Previous studies have suggested that citrinin exposure increases abnormal ER distribution in mice (Sun et al, 2020).…”

“…We also assessed the distribution of the Golgi apparatus using Golgi-Tracker in porcine oocytes post-IVM. The distribution of the Golgi apparatus is connected with the distribution of the ER and CGs, and this organelle is generally localized in the cortex of MII oocytes (De los Reyes et al, 2012). Therefore, we classified the distribution of the Golgi apparatus into two types.…”

Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes

“…Cumulus oocytes complexes from all six groups were subjected to IVM at 38°C, 5% CO 2 and saturated humidity for 72 hr (De los Reyes, Palomino, Jofré, Villarroel, & Moreno, ). Experiments were conducted using 100 μl droplets of each medium covered with mineral oil (Sigma), each containing no more than eight COCs.…”

Luteolin Orchestrates Porcine Oocyte Meiotic Progression by Maintaining Organelle Dynamics Under Oxidative Stress

“…Cell organelles including mitochondria, the endoplasmic reticulum (ER), and microtubules play important roles in both nuclear and cytoplasmic maturation through the regulation of protein and ATP synthesis and chromosome segregation ( Mao et al, 2014 ). However, inadequate in vitro conditions lead to the abnormal behavior of cell organelles and finally result in disrupted meiotic maturation ( Ueno et al, 2005 ; De los Reyes et al, 2011 ; De los Reyes et al, 2012 ).…”