The enzyme catalyzed polymerization of luminol (5-amino-2, 3-dihydroxy-1, 4-phthalazinedione) in the presence of polystyrenesulphonate (SPS) is introduced. Polymerization was achieved using horseradish peroxidase (HRP) as a biocatalyst in a phosphate buffer at pH 8. A green, water-soluble conducting PLUM/SPS complex was obtained. The physico-chemical properties of the product, including possible photoluminescence of the PLUM/SPS complex, were studied. The PLUM/SPS complex was shown to be a chemiluminescent (CL) material that is highly sensitive towards Fe 3+ ions. The quantum yield of the PLUM chemiluminescence was determined to be moderate (0.09 ± 0.01) compared to other chemiluminescent compounds. UV-visible, FTIR, 1 H NMR, GPC and conductivity measurements confirmed that the electroactive form of polyluminol (PLUM) was formed and complexed to the SPS.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.