The data present in this article is associated with influence of piperine (secondary metabolite) on the antihyperlipidemic and antioxidant activity of methanolic extract of Tagetes patula (METP). METP was evaluated for antihyperlipidemic and antioxidant potential. Phytoconstituents of METP were identified using gas chromatography linked with a mass spectrometer. in vivo antihyperlipidemic activity of METP at the dose of 200 and 400 mg/kg b. wt. and 200 and 400 mg/kg b. wt. along with piperine (20 mg/kg b. wt.) were evaluated by Propylthiouracil induced and Triton X-100 induced hyperlipidemia in rats. Propylthiouracil significantly increased the serum TC (p<0.01), TG (p<0.01), LDL (p<0.01) and VLDL (p<0.01) levels and induction of HDL (p<0.01) at a dose of 400 mg/kg b. wt. along with piperine. Triton X-100 at a single dose of i.p increased lipid levels within 48 h. Increased lipid levels were significantly reduced TC (p<0.01), TG (p<0.01), LDL (p<0.05) and VLDL (p<0.05) by METP at doses of 200 and 400 mg/kg b. wt. along with piperine. Current data were also supported by histological study of livers, Cord pattern of hepatocytes, few periportal lymphocytes in focal area observed in hyperlipidemic rats and hepatocyte, periportal and centrilobular region of liver appear normal in treated group. METP along with piperine (capability to enhance bioavailability and has a property of increasing oral absorption of drugs) showed promising antioxidant and antihyperlipidemic activity which suggests the further use of Tagetes patula extract for the management of hyperlipidemia and atherosclerosis.
The data present in this article is related to evaluation of standardized methanolic extract of Vernonia elaeagnifolia aerial parts [MEVE] , a species of Asteraceae family for antiulcer potential. Antiulcer activity of MEVE (200 and 400 mg/kg, b.w., p.o. ) was evaluated with ethanol and aspirin induced ulcer models and pylorus ligation induced gastric ulcer model. The antioxidant potential of MEVE was evaluated with nitric oxide radicals, hydroxyl radical and H 2 O 2 radical scavenging assay against standard ascorbic acid to correlate antioxidant and antiulcerogenic action. MEVE significantly protects the gastric mucosa against the ethanol and aspirin induced ulcer and pylorus ligation induced ulcer challenge. MEVE had shown significant [normal control: p < 0.0001, disease control: p < 0.0001, standard: p < 0.0001] decrease in the ulcer index produced by all three models in rats as compared to the standard drug omeprazole [20 mg/kg, b.w., p.o. ]. The present data suggest that aerial parts of Vernonia elaeagnifolia possess significant antiulcer activity, which may attributed to its antioxidant mechanism of action.
Objectives: To study HPTLC densitometric quantification of kaempferol in aqueous extract fraction (EN6) of leaves of Euphorbia neriifolia Linn. Methods: Chromatographic method was employed on pre-washed and pre-activated 10.0 x 10.0 cm aluminum Lichrosphere HPTLC plates pre-coated by silica gel 60 F 254 of 0.2 mm thickness layer as a stationary phase. Solvent system toluene: ethyl acetate: formic acid (6:4:1 v/v/v) was used. Densitometric analysis of kaempferol was carried out at 254 nm in absorptionreflectance mode. Percent content of kaempferol was quantified using proposed HPTLC densitometric method. The method was validated by ICH guidelines for its linear range, linearity, LOD and LOQ, specificity, precision, reproducibility and recovery studies. Results: HPTLC finger printing analysis showed that R f value of peak of kaempferol (Rf 0.91) was closely compacted with aqueous extract fraction (EN6; R f 0.90) that indicates identification of kaempferol. Percent content of kaempferol was quantified and found to be 0.024817% in aqueous extract fraction (EN6) for 10 µl volume applied. Linear range was found100-600 ng/spot with correlation coefficient 0.99834. LOD and LOQ were found 100 and 300 ng. Proposed method was specific. The % CV for intra-day was 2.422 and inter-day was 2.522 at concentration of 400 ng/spot. The % CV was found 2.1, hence the method was reproducible. Average percent recovery was 95.57% in aqueous extract fraction (EN6), therefore, the method was found to be accurate. Conclusion: Developed HPTLC method was found to be simple, specific, precise, reproducible and accurate.Here, the first time we have reported HPTLC method for kaempferol in aqueous extract fraction (EN6). However, there is a requirement to carry forward these studies by using some other standard markers in aqueous extract fraction (EN6) of E. neriifolia leaves for exploring its phyto-constituents that could have therapeutic potential in various diseases.
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