The sensitivity of field isolates of STEC O157, O26 and reference strains from collections to different concentrations of sodium hypochlorite, sodium benzensulfochloramid (chloramine B), glutaraldehyde with glyoxal, peracetic acid and lactic acid was verified. The most effective disinfectant was peracetic acid at the concentration of 0.02%, followed by chloramine B and lactic acid at the concentration of 0.5%. The field isolate of STEC O157 in comparison with the other tested strains of E. coli was more resistant to the activity of peracetic both and lactic acid and to chlorine releasing disinfectants. Its resistance was comparable to that of reference E. coli strain CNCTC 301/60, which is the reference micro-organism for efficacy testing of disinfectants. Statistically significant difference among the individual tested strains was not found. Differences between the tested STEC strains and reference strains in evaluation of growth curves were shown. STEC O157 and O26, more than the other tested strains, showed a higher resistance to acid environment in this characteristic. The reference E. coli strain CNCTC 301/60 and field isolate STEC E. coli O157 were the most resistant of all the tested strains, reference non toxigenic E. coli O157 strain was the most resistant to aldehydes.