Objective: The aim of the present study was to screen phytochemical constituents and evaluate antimicrobial and synergistic antimicrobial properties of leaves and stem of Hibiscus sabdariffa. Methods: The extraction was done by cold maceration method using 80% aqueous methanol. The antimicrobial efficacy and synergistic antimicrobial activity were carried out by disc diffusion assay against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Results: Phytochemical analysis revealed the presence of carbohydrate, protein, alkaloids, phytosterols, flavonoids, and diterpenes in both the leaves and stem extracts while saponins, phenol, and tannins were found to be present only in the leaf extract. Both the extracts inhibited the tested bacteria with minimum inhibitory concentration value of 10 mg/ml. Aqueous methanolic extract of leaf showed higher antibacterial activity against S. aureus and P. aeruginosa and also exhibited synergistic activity with the antibiotic chloramphenicol against S. aureus. Conclusion: The present study concludes that H. sabdariffa is a potential source of bioactive components and also provides information on synergistic activity of leaf extract. The results can contribute to the development of potent antibacterial agents.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.