Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.
Multi-drug resistant (MDR) bacteria are becoming a worldwide problem due to limited options for treatment. Moreover, patients infected by MDR with highly virulent accessories are worsening the symptoms, even to the point of causing death. In this study, we isolated bacteria from 14 inanimate surfaces that could potentially be reservoirs for the spread of bacterial infections in the medical university. Blood agar media was used for bacterial isolation. The bacterial colony that showed hemolytic activities on each surface was tested for antimicrobial susceptibility against eight different antibiotics. We found that MDR bacterium, namely TB1, which was isolated from a toilet bowl, was non-susceptible to ampicillin, imipenem, chloramphenicol, amoxicillin-clavulanic acid, gentamicin, and tetracycline. Another MDR bacterium isolated from the mobile phone screen of security officers, namely HSO, was resistant to chloramphenicol, gentamicin, tetracycline, and cefixime. An in vivo virulence test of bacterial isolates used Omphisa fuscidentalis larvae as an alternative to Galleria mellonella larvae for the infection model. A virulence test of TB1 in O. fuscidentalis larvae revealed 20% survival in the bacterial density of 104 and 105 CFU/larvae; and 0% survival in the bacterial density of 106 CFU/larvae at 24 h after injection. Bacterial identification was performed for TB1 as a potential virulent isolate. Bacterial identification using partial 16s rRNA gene showed that TB1 exhibited 99.84% identity to Escherichia fergusonii 2611. This study concludes that TB1 is a potentially virulent MDR E. fergusonii isolated from toilet bowls at a medical university.
Pendahuluan: Penyakit infeksi pernafasan karena bakteri merupakan penyakit yang memiliki kasus tinggi di Indonesia. Penyakit pernafasan karena infeksi bakteri juga bersifat nosokomial dan dapat menyebar di komunitas. Bakteri yang menyebabkan infeksi pernafasan ini sangat beragam baik dari jenis bakterinya, tingkat resistensinya, maupun tingkat virulensinya. Tingkat virulensi bakteri mempengaruhi konsekuensi penyakitnya pada pasien. Metode: Dalam menentukan virulensi bakteri secara in vivo, beberapa hewan dapat digunakan sebagai model infeksi pernafasan karena bakteri seperti tikus, ikan zebra (Danio rerio), ngengat lilin (Galleria mellonella), nematoda Caenorhabditis elegans. Hasil: Dari sudut pandang author, jika menilik biaya dan kemudahan sebagai prioritas, ulat G. mellonella memiliki beberapa keunggulan dibandingkan hewan lain seperti biaya produksi murah, tidak membutuhkan perizinan etik, dapat diinkubasi pada suhu 37° C, juga sudah banyak publikasi yang menggunakan ulat ini dalam uji virulensi bakteri. Kesimpulan: Review artikel ini akan menjelaskan perbandingan kelebihan dan kekurangan hewan model tersebut dalam model in vivo bakteri infeksi pernafasan.
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