Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria

Abstract

Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.

Virulence Evaluation of Aeromonas spp. KS-1 Isolated from Kitchen Sponge using Omphisa fuscidentalis Larvae

“…Kitchen sponge can disseminate the bacteria as the residue of cleaning the food utensils that can be the factor of bacterial cross contamination of foodborne transmitted infections (Wolde and Bacha 2016). Inappropriate handling, improper storage, and long duration usage, lead to survival and growth of microbial in the kitchen sponge, including E. coli, Staphylococcus spp., Salmonella spp., Klebsiella spp., Enterobacter cloacae, and Cronobacter sakazakii (Rossvoll et al 2015;Cardinale et al 2017;Marotta et al 2018;Inggraini et al 2021;Ilsan et al 2022). Although Aeromonas spp.…”

The antibacterial and antibiofilm potential of Paederia foetida Linn. leaves extract

“…Commonly, microbial infections are treated with antibiotics. However, unfortunately, this has been increasingly complicated because some pathogenic bacteria having become more resistant to several antibiotics (Inggraini et al, 2021). Therefore, the finding of a new antimicrobial agent is a highly important subject to study.…”