This research is classified as developmental research that aims to determine (1) validity, (2) practicality, (3) effectiveness. This research is a 4-D model development research. The subjects of this research are students of class XI of SMA Negeri 2 Gorontalo in the 2021/2022 academic year. The data collection techniques used are instrument, response questionnaire, and test. The findings indicate that the avaluation instrument developed can be used in the learning process, and it is observable from the percentage of validation result, namely, validation from material expert for 95.5%, evaluation tool expert for 86.6%, application expert for 94.0%, and practitioner for 95.0% with a very feasible category. Additionally, the practicality of the evaluation instrument is noticeable from the implementation of teacher activities, student activities, and student responses, which are 100%, 91.5%, and 92.0% respectively, with very good categories. In addition, the effectiveness of evaluation instrument can be identified from the result of analysis of learning outcomes using t-test and N-Gain score. The t-test Sig. (2-failed) of 0.000 indicates the existence of significant difference while the N-Gain score with a value of 0.66 is in the medium category. Thus, the evaluation instrument developed using Kahoot application is feasible and can be used in Biology learning for immune system topic for class XI students of SMA Negeri 2 Gorontalo
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