Laser-Induced Localized Growth of Methylammonium Lead Halide Perovskite Nano-and Microcrystals on SubstratesStable MAPbBr 3 crystals with different sizes are successfully localized on a flat substrate via laser-induced heating of the liquid precursors. By adjusting the infrared laser parameters, luminescent arrays and photoconductive wires are grown on-site. This technique can be used to guide the writing of other patterns for specific functionalities.
Fluorescence microscopy and derived techniques are continuously looking for photodetectors able to guarantee increased sensitivity, high spatial and temporal resolution and ease of integration into modern microscopy architectures. Recent advances in single-photon avalanche diodes (SPADs) fabricated with industry-standard microelectronic processes allow the development of new detection systems tailored to address the requirements of advanced imaging techniques (such as image-scanning microscopy). To this aim, we present the complete design and characterization of two bidimensional SPAD arrays composed of 25 fully independent and asynchronously-operated pixels, both having fill-factor of about 50% and specifically designed for being integrated into existing laser scanning microscopes. We used two different microelectronics technologies to fabricate our detectors: the first technology exhibiting very low noise (roughly 200 dark counts per second at room temperature), and the second one showing enhanced detection efficiency (more than 60% at a wavelength of 500 nm). Starting from the silicon-level device structures and moving towards the in-pixel and readout electronics description, we present performance assessments and comparisons between the two detectors. Images of a biological sample acquired after their integration into our custom imagescanning microscope finally demonstrate their exquisite on-field performance in terms of spatial resolution and contrast enhancement. We envisage that this work can trigger the development of a new class of SPAD-based detector arrays able to substitute the typical singleelement sensor used in fluorescence laser scanning microscopy.
We report a laser-based approach for the fast fabrication of high-optical-quality polymeric microlenses and microlens arrays with controllable geometry and size. Our strategy consists of the direct laser printing of microdroplets of a highly viscous UV prepolymer at targeted positions, followed by photocuring. We study the morphological characteristics and imaging performance of the microlenses as a function of the substrate and laser parameters and investigate optimal printing conditions and printing mechanisms. We show that the microlens size and focusing properties can be easily tuned by the laser pulse energy, with minimum volumes below 20 fL and focal lengths ranging from 7 to 50 μm.
In today's fast‐paced and well‐connected world, consumer electronics are evolving rapidly. As a result, the amount of discarded electronic devices is becoming a major health and environmental concern. The rapid expansion of flexible electronics has the potential to transform consumer electronic devices from rigid phones and tablets to robust wearable devices. This means increased use of plastics in consumer electronics and the potential to generate more persistent plastic waste for the environment. Hence, today, the need for flexible biodegradable electronics is at the forefront of minimizing the mounting pile of global electronic waste. A “bioadvantaged” approach to develop a biodegradable, flexible, and application‐adaptable electronic components based on crop components and graphene is reported. More specifically, by combining zein, a corn‐derived protein, and aleuritic acid, a major monomer of tomato cuticles and sheellac, along with graphene, biocomposite conductors having low electrical resistance (≈10 Ω sq−1) with exceptional mechanical and fatigue resilience are fabricated. Further, a number of high‐performance electronic applications, such as THz electromagnetic shielding, flexible GHz antenna construction, and flexible solar cell electrode, are demonstrated. Excellent performance results are measured from each application comparable to conventional nondegrading counterparts, thus paving the way for the concept of “plant‐e‐tronics” towards sustainability.
Three-dimensional imaging at high-spatiotemporal resolutions and over large penetration depths is key for unmasking the dynamics and structural organization of complex biological systems. However, the need to axially shift the focus, with consequent limitations in imaging speed, and signal degradation at large depths due to scattering effects, makes this task challenging. Here, we present a novel approach in 2-photon excitation microscopy that allows fast volumetric imaging and enhanced signal-to-background (S/B) in thick tissue. Our technique is based on ultrafast beam shaping at each pixel by means of an acoustic optofluidic lens. Shaping the excitation beam with different phase profiles enables both high-speed axial focus shifting, for continuous volumetric imaging, and controlled aberrated imaging, advantageous for out-of-focus background removal. We provide a theoretical description of our approach, and demonstrate volumetric imaging of neuronal cells from a mouse brain slice with enhancements in S/B up to a factor of 10 over a depth of 600 μm.
To date, the feasibility of super-resolution microscopy for imaging live and thick samples is still limited. Stimulated emission depletion (STED) microscopy requires high-intensity illumination to achieve sub-diffraction resolution, potentially introducing photodamage to live specimens. Moreover, the out-of-focus background may degrade the signal stemming from the focal plane. Here, we propose a new method to mitigate these limitations without drawbacks. First, we enhance a STED microscope with a detector array, enabling image scanning microscopy (ISM). Therefore, we implement STED-ISM, a method that exploits the working principle of ISM to reduce the depletion intensity and achieve a target resolution. Later, we develop Focus-ISM, a strategy to improve the optical sectioning and remove the background of any ISM-based imaging technique, with or without a STED beam. The proposed approach requires minimal architectural changes to a conventional microscope but provides substantial advantages for live and thick sample imaging.
During plasma excitation by high power Alfven waves at TCA, signals at harmonics of the generator frequency are observed in the plasma scrape-off layer. Experimental investigations of the sheath effect and the excitation efficiency and dispersion properties of the harmonics are reported. The results indicate that the harmonics arise, either directly or indirectly, through the driven Alfven waves and not through the sheath effect at the exciting antenna. The RF ion saturation current is observed to have a nonnegligible peak amplitude in comparison with the time averaged ion saturation current and may provide evidence of a nonlinear evolution of the driven Alfven waves
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.