HPV oncoproteins can modulate DNMT1 expression and activity, and previous studies have reported both gene-specific and global DNA methylation alterations according to HPV status in head and neck cancer. However, validation of these findings and a more detailed analysis of the transposable elements (TEs) are still missing. Here we performed pyrosequencing to evaluate a 5-CpG methylation signature and Line1 methylation in an oropharyngeal squamous cell carcinoma (OPSCC) cohort. We further evaluated the methylation levels of the TEs, their correlation with gene expression and their impact on overall survival (OS) using the TCGA cohort. In our dataset, the 5-CpG signature distinguished HPV-positive and HPV-negative OPSCC with 66.67% sensitivity and 84.33% specificity. Line1 methylation levels were higher in HPV-positive cases. In the TCGA cohort, Line1, Alu and long terminal repeats (LTRs) showed hypermethylation in a frequency of 60.5%, 58.9% and 92.3%, respectively. ZNF541 and CCNL1 higher expression was observed in HPV-positive OPSCC, correlated with lower methylation levels of promoter-associated Alu and LTR, respectively, and independently associated with better OS. Based on our findings, we may conclude that a 5-CpG methylation signature can discriminate OPSCC according to HPV status with high accuracy and TEs are differentially methylated and may regulate gene expression in HPV-positive OPSCC.
Hemophilic pseudotumors are rare, but well known complications of severe hemophilia A, which most frequently develops at the femur, tibia, pelvic bones, iliac bones, or rarely in the cranium or gnathic bones. This report describes a case of hemophilic pseudotumor of the maxillary alveolar ridge that occurred in a boy with mild hemophilia A (14% factor VIII activity). The lesion, which presented as an alveolar mucosal swelling, responded well to enucleation, curettage, and intralesional fibrin glue injection.
Objective
To report the clinicopathologic features of acquired oral syphilis cases in South American countries.
Materials and Methods
Clinical data were retrospectively collected from the records of 18 oral diagnostic services in Argentina, Brazil, Chile, Colombia, Venezuela, Uruguay, and Peru. Serologies of nontreponemal and treponemal tests were used for diagnosis.
Results
The series comprised 339 cases of acquired oral syphilis. Secondary syphilis ranked as the most common stage (86.7%). Lesions were more frequent among males (58.0%) and young adults with a mean age of 33.3 years. Individuals aged 20–29 years were most affected (35.3%). The most commonly involved sites were the tongue (31.6%), lip/labial commissure (25.1%), and hard/soft palate (20.4%). Clinically, acquired oral syphilis usually presented as mucous patches (28.4%), papules (25.7%), and ulcers (18.1%). Skin manifestations occurred in 67.7% of individuals, while lymphadenopathy and fever were observed in 61.3% and 11.6% of all subjects, respectively. Most patients were treated with the benzathine penicillin G antibiotic.
Conclusion
This report validates the spread of acquired oral syphilis infection among young adults in South America. Our directives include accessible diagnostic tools for proper disease screening, surveillance, and counselling of affected individuals, especially in low‐ and middle‐income countries.
Introdução: O ameloblastoma é um tumor odontogênico de origem epitelial que, pela freqüência, subtipos clínicos, grande capacidade infiltrativa e alta tendência à recorrência tem merecido bastante atenção. Objetivo: Realizar estudo retrospectivo dos casos diagnosticados histopatologicamente de ameloblastoma no Serviço de Anatomia Patológica do Hospital Universitário Antônio Pedro da Universidade Federal Fluminense (HUAP/UFF) no período de 1997 a 2007. Material e métodos: Foram selecionados casos de ameloblastoma correspondentes ao período da pesquisa, e dados como idade, sexo, raça, localização da lesão, aspecto radiográfico, tipo de biópsia, diagnósticos clínico e histopatológico foram compilados das fichas de requisição e/ou prontuários. Resultados: Depois do levantamento e da revisão das lâminas para confirmação do diagnóstico de ameloblastoma, a amostra totalizada foi de 23 casos. Os pacientes mais acometidos eram do sexo masculino, leucodermas e estavam entre a primeira e a terceira década de vida. Todos os casos ocorreram na mandíbula, sendo a região posterior a mais afetada. Predominaram o aspecto radiográfico multilocular e, histopatologicamente, o padrão multicístico, sendo os subtipos folicular e híbrido os mais freqüentes. Não foram observados casos de ameloblastoma desmoplásico e de células basais. Conclusões: A freqüência do ameloblastoma varia de acordo com a região geográfica avaliada, e a realização de estudos retrospectivos pode contribuir para a identificação de grupos e dos possíveis fatores associados ao seu desenvolvimento.
OBJETIVO: Fazer um estudo retrospectivo, correlacionando os diagnósticos citopatológicos obtidos por punção aspirativa por agulha fina (PAAF) com os diagnósticos histopatológicos de lesões da região bucomaxilofacial, de pacientes que foram atendidos no Serviço de Cirurgia e Traumatologia Bucomaxilofacial do Hospital Universitário Antonio Pedro/UFF, entre maio de 1999 e maio de 2004. MÉTODO: Foram analisados os laudos cito e histopatológicos emitidos pelo Serviço de Anatomia Patológica (SAP-HUAP), de quarenta e cinco pacientes submetidos à a punção aspirativa por agulha fina (PAAF) por apresentar lesão na região bucomaxilofacial. RESULTADOS: Foi obtida uma precisão diagnóstica da citopatologia a partir da PAAF de 77,8% (35 casos), e especificidade e sensibilidade da técnica de 100%, não havendo nenhum resultado falso-positivo e falso-negativo. CONCLUSÃO: A citopatologia realizada a partir da PAAF é um método diagnóstico inicial eficiente, de baixo custo, praticamente indolor e rápido de ser realizado, que contribui para a identificação da natureza da lesão proporcionando um melhor planejamento terapêutico.
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