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stress relief techniques, including the Chamber restricted environment stimulation technique (REST), are becoming increasingly popular in Slovakia. Given this, it is necessary to investigate this method in an experimental setting. According to Urbiš (2012), dark therapy could be a suitable method for people living under constant stress, tension, and difficult living conditions and can also be used as training in coping with stressful situations. The focus of this study was to assess the relationship between a short‑term Chamber REST and perceived stress. Our research sample consisted of 42 participants who participated in a three‑hour stay in the laboratory of REST at Comenius University. Participants were psychology students. The selection of the research sample and length of stay was mainly based on the COVID-19 pandemic virus. The average age of the participants was 25 years (SD = 5.63). In terms of gender, 32 women (76.19%) and 10 men (23.81%) participated in the research. 21 respondents (50%) stated that they had some previous experience (not a one‑time, but repeated) with relaxation techniques. 20 participants (47.62%) had no prior experience. We measured experienced stress using the Perceived Stress Scale (PSS, Cohen et al., 1983) two days before the stay and one week after, and stress and arousal using The Stress Arousal Checklist (SACL, Mackay et al., 1978) before and after stay. The results show that the difference in perceived stress decreased significantly right after short‑term Chamber REST (p = 0.001; W = 556.00) and even one week after the stay (p = 0,016; W=0,932). There were no significant changes in the SDNN parameter before staying in the dark (M = 65.46, SD = 53.79) and after (M = 73.57, SD = 31.14) Wilk’s Lambda = .939, F (1.30) = 1.947, p = .173. No significant changes are also in the rMSSD index before (M = 62.45, SD = 66,42) and after (M = 70.57, SD = 43.80) Wilk’s Lambda = .961, F (1,30) = 1.214, p =.790; neither in HF before (M = 3 307.12, SD = 7 524.92) and after (M = 2 384.77, SD = 2 934.18) Wilk’s Lambda =.995, F (1,30) =.140, p =.711. There was not a significant effect of gender, Wilk’s Lambda =.991, F (1,30) =.283, p =.599 or previous experiences, Wilk’s Lambda = 1.000, F (1,30) =.001, p =.980 on SDNN. We also did not observe a significant effect of gender, Wilk’s Lambda =.996, F (1,30) =.133, p =.717 or previous experiences, Wilk’s Lambda = 1.000, F (1,30) = .014, p = .907 on rMSSD. The same results are in the HF index, with no significant effects of gender, Wilk’s Lambda = 0.985, F (1,30) = .469, p = .499 and previous experiences with relaxation methods Wilk’s Lambda = 1.000, F (1,30) = .000, p = .994. According to our expectations, staying in the dark should reduce the level of stress experienced. The results showed the statistical significance of the difference in the stress using PSS and SACL questionnaires scores before and after dark stay. Participants scored lower after their stay in the dark than before, which is in line with our expectations accord‑ ing to other authors (Malůš et al., 2016; Suedfeld & Borrie, 1999) and highlights the potential of Chamber REST to reduce the level of experienced stress. Based on HRV analysis, we conclude that there was a change in measured HRV indexes, however, the changes were not significant. We observed an increase in heart rate variability (SDNN, RMSSD) indicating an increase in parasympathetic tone, compatible with elevated relaxation. Gender factors and previous experience with relaxation methods have no effect. In conclusion, we found the difference in perceived stress decreased significantly right after short‑term Chamber REST and one week after the stay. An increase in heart rate variability (SDNN, RMSSD) after the stay indicated an increase in parasympathetic tone, however, the changes were not significant. There was no effect of factors of gender and previous experience with relaxation methods.

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Background The COVID-19 pandemic is a serious and long-term stressor for healthcare workers, therefore hair cortisol concentrations (HCCs) could be used to assess this prolonged stress. Method HCCs of 67 female nurses were measured during spring 2020, with a follow-up performed in autumn 2020 with 57 participants. Both times, HCCs were analyzed from two 3 cm hair segments corresponding to periods of hair growth before and during the spring wave of the pandemic, during the summer release, and the autumn wave of the pandemic. Results Data from spring 2020, showed higher HCCs in hair grown during the pandemic compared to older hair (90%CI η 2 =0.123-0.397), while nurses reporting a high risk of infection (n=33) had higher HCCs (90%CI η 2 =0.002-0.176) than nurses reporting low risk (n=34). In the follow-up, hair samples corresponding to worsening of the pandemic had higher HCCs than samples from summer release (90%CI η 2 =0.002-0.201). Three groups were compared instead of two, as only 9 nurses reported low risk, 25 high risk, and 23 daily work with COVID-19 patients. Group differences were not significant. Conclusion Data from the follow-up supported the findings of higher HCCs in nurses during the worsening of the pandemic.