Background:The timing of wounds production is a significant issue in forensic pathology. Although various methods have been evaluated, obtaining an accurate dating of lesions is still a challenge. The pathologist uses many parameters to value wound age, such as histological and immunohistochemical. In recent years, there have been many studies regarding the use of miRNAs in wound-age estimation; indeed, miRNAs have multiple potential uses in forensic pathology.Scope:This review aims to verify the efficacy and feasibility of miRNAs as a tool for determining the timing of lesions.Materials and Methods:The authors conducted the systematic review according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. PubMed was used as a search engine to find articles published between January, 1st 2016 and October, 1st 2021, to evaluate the current state of the art regarding wound-age estimation.Results:A total of 256 articles were collected; after screening according to PRISMA guidelines, the systematic review included 8 articles. The studies included in this review were all Original articles evaluating the use of biomarkers for wound-age determination.Discussion and Conclusion:The literature review showed that analysis of miRNA is an innovative field of study with significant potentiality in forensic pathology. There are few studies, and almost all of them are at an early stage. The challenge is to understand how to standardize the samples' selection to obtain reliable experimental data. This observation represents a necessary prerequisite to planning further clinical trials.
Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aims to investigate anti-GPA immunohistochemical staining in order to evaluate the vitality of the lesion from corpses in different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by the Institute of Legal Medicine of Rome in 2010–2018: four samples of fractured bones and three samples of soft tissues. For the control case, the fracture region of the femur was sampled. The results of the present study confirm the preliminary results of other studies, remarking the importance of the GPA immunohistochemical staining to highlight signs of survival. Moreover, this study suggests that the use of this technique should be routinely applied in cases of corpses with advanced putrefaction phenomena, even when the radiological investigation is performed, the macroscopic investigation is inconclusive, the H&E staining is not reliable. This experimental application demonstrated that the use of monoclonal antibody anti-human GPA on bone fractures and soft tissues could be important to verify whether the lesion is vital or not.
According to WHO data, suicide is a public health priority. In particular, suicide is the fourth-leading cause of death in young people. Many risk factors of suicide are described, including individual-, relationship-, community-, and societal-linked ones. The leading factor is the diagnosis of mental illness. Nevertheless, not all people who attempt suicide are psychiatric patients; these characteristics help define high-risk populations. There are currently no useful biomarkers to indicate the risk of suicide. In recent years, neurotrophic factors have increasingly become of scientific interest. This review aims to summarize the current scientific knowledge on the correlation between BDNF and GDNF and suicide, to theorize whether neurotrophins could be a reliable marker for an early diagnosis of suicidal risk. The authors conducted a systematic review following PRISMA criteria. They found eight research papers in agreement with the inclusion criteria. According to the results of these studies, there may be a connection between BDNF brain levels and complete suicide, although there are discrepancies. A lack of interest in GDNF may suggest less involvement in the suicidal dynamic. Further studies may provide helpful information to researchers.
Introduction: Suicide by abdominal cut wounds and consequent disembowelment is a modality rarely described in the literature, with rates between 1.6% and 3%. The incidence is higher in men. This type of suicide might be so unusual as the abdominal injuries are not supposed to be related to immediate death (compared to the wrists or throat, which involves rapid bleeding, for example). Considering the infrequency of suicides by abdominal self-cutting, in such cases, the main hypothesis is a homicide, especially in those with multiple injuries or occurred in a complex setting. These cases require a detailed autopsy report with the analysis of the injuries and circumstances of death (e.g., farewell note, history of depression, previous suicide attempts, defense injuries, and signs of hesitation) to allow a differential diagnosis between suicide and homicide. This study aims to highlight the characteristics of suicides through self-cut wounding, focusing on those that determine a large abdominal opening and evisceration. Methods: The authors conducted the literature search using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, on PubMed and Scopus databases, using the following keywords: "(suicide) AND (abdominal stab wounds)", "(suicide) AND (abdominal sharp injuries)", "(suicide) AND (abdominal self-stabbing)", "(suicide) AND (abdominal cut wounds)". Results: 7 articles were included in the systematic review, for a total of 11 cases of suicide by abdominal self-cutting. Of these cases, 3 were women and 8 were men. Conclusions: The analysis of the external examination findings versus the crime scene results is essential to clarify if injuries are self-inflicted with suicidal intention. Suicide through a violent act can also be practiced by people who have no history of psychiatric conditions or other risk factors. Therefore, in cases of abdominal cut and evisceration with victims' survival time, the forensic pathologist has to consider that the deceased himself could cause modifications to the crime scene, turning it into a complicated suicide.
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