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Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33 -29.80 %) involving chromatin transfer among 2 -3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and broad cytoplasmic channels from early prophase to tetrad stage. However, frequency of its occurrence during the later meiotic stages is rather low. Chromatin transfer results into the formation of hypo-, hyperploid and enucleated PMCs. Various meiotic abnormalities associated with cytomixis such as chromatin stickiness, pycnotic chromatin, interbivalent connections, out of plate bivalents, late disjunction of bivalents, and laggards and bridges resulted into some pollen sterility (16.33 -49.30 %) and heterogeneous pollen grains size.

BackgroundHemicelluloses are a diverse group of complex, non-cellulosic polysaccharides, which constitute approximately one-third of the plant cell wall and find use as dietary fibres, food additives and raw materials for biofuels. Genes involved in hemicellulose synthesis have not been extensively studied in small grain cereals.ResultsIn efforts to isolate the sequences for the cellulose synthase-like (Csl) gene family from wheat, we identified 108 genes (hereafter referred to as TaCsl). Each gene was represented by two to three homeoalleles, which are named as TaCslXY_ZA, TaCslXY_ZB, or TaCslXY_ZD, where X denotes the Csl subfamily, Y the gene number and Z the wheat chromosome where it is located. A quarter of these genes were predicted to have 2 to 3 splice variants, resulting in a total of 137 putative translated products. Approximately 45% of TaCsl genes were located on chromosomes 2 and 3. Sequences from the subfamilies C and D were interspersed between the dicots and grasses but those from subfamily A clustered within each group of plants. Proximity of the dicot-specific subfamilies B and G, to the grass-specific subfamilies H and J, respectively, points to their common origin. In silico expression analysis in different tissues revealed that most of the genes were expressed ubiquitously and some were tissue-specific. More than half of the genes had introns in phase 0, one-third in phase 2, and a few in phase 1.ConclusionDetailed characterization of the wheat Csl genes has enhanced the understanding of their structural, functional, and evolutionary features. This information will be helpful in designing experiments for genetic manipulation of hemicellulose synthesis with the goal of developing improved cultivars for biofuel production and increased tolerance against various stresses.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1142-z) contains supplementary material, which is available to authorized users.

Cellulose is the primary determinant of mechanical strength in plant tissues. Late-season lodging is inversely related to the amount of cellulose in a unit length of the stem. Wheat is the most widely grown of all the crops globally, yet information on its CesA gene family is limited. We have identified 22 CesA genes from bread wheat, which include homoeologs from each of the three genomes, and named them as TaCesAXA, TaCesAXB or TaCesAXD, where X denotes the gene number and the last suffix stands for the respective genome. Sequence analyses of the CESA proteins from wheat and their orthologs from barley, maize, rice, and several dicot species (Arabidopsis, beet, cotton, poplar, potato, rose gum and soybean) revealed motifs unique to monocots (Poales) or dicots. Novel structural motifs CQIC and SVICEXWFA were identified, which distinguished the CESAs involved in the formation of primary and secondary cell wall (PCW and SCW) in all the species. We also identified several new motifs specific to monocots or dicots. The conserved motifs identified in this study possibly play functional roles specific to PCW or SCW formation. The new insights from this study advance our knowledge about the structure, function and evolution of the CesA family in plants in general and wheat in particular. This information will be useful in improving culm strength to reduce lodging or alter wall composition to improve biofuel production.

Field pea (Pisum sativum L.) is an important protein-rich pulse crop produced globally. Increasing the lipid content of Pisum seeds through conventional and contemporary molecular breeding tools may bring added value to the crop. However, knowledge about genetic diversity and lipid content in field pea is limited. An understanding of genetic diversity and population structure in diverse germplasm is important and a prerequisite for genetic dissection of complex characteristics and marker-trait associations. Fifty polymorphic microsatellite markers detecting a total of 207 alleles were used to obtain information on genetic diversity, population structure and marker-trait associations. Cluster analysis was performed using UPGMA to construct a dendrogram from a pairwise similarity matrix. Pea genotypes were divided into five major clusters. A model-based population structure analysis divided the pea accessions into four groups. Percentage lipid content in 35 diverse pea accessions was used to find potential associations with the SSR markers. Markers AD73, D21, and AA5 were significantly associated with lipid content using a mixed linear model (MLM) taking population structure (Q) and relative kinship (K) into account. The results of this preliminary study suggested that the population could be used for marker-trait association mapping studies.

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Summary Chromosomal studies on Clematis montana from Dalhousie hills, Himachal Pradesh, India have led to the identification of an accession that exhibited aberrant male meiosis, pollen sterility and heterogeneous sized fertile pollen grains. The accession, which exists at diploid level (nϭ8), depicts the phenomenon of cytomixis involving chromatin transfer among proximate meiocytes (PMs). The chromatin transfer in the PMCs has also caused various meiotic irregularities like chromatin stickiness, interbivalent connections, unorganized and pycnotic chromatin, laggards/ chromatin bridges during anaphases/telophases, micronuclei and included micronuclei in microspores, triads and polyads. Consequent to cytomixis and associated meiotic irregularities, the products of PMCs result in some pollen sterility and variable sized fertile pollen grains. Though the Cytological status of such variable sized pollen grains could not be ascertained at present, the large sized pollen grains, which are almost double sized, may be of '2n' constitution and play an important role in the origin of intraspecific polyploidy in the species. The small sized fertile pollen grains which are the products of hypoploid PMCs could lead to the origin of aneuploids. Similar effects of cytomixis on meiotic course resulting in pollen malformation have been reported earlier in a number of species by the authors from this laboratory.

Potato (Solanum tuberosum) is one of four major food crops in the world. Weed control is a major component in potato production and has been accomplished using different methods, including but not limited to the use of herbicides and straw mulch. A combination of preemergence herbicide and straw mulch may improve weed control; however, no information is available for combining both methods, along with their effects on weed control, weed density, and potato tuber yields. The objective of this study was to evaluate weed control in potato using atrazine or straw mulch applied alone at different rates or in combination. A field experiment was conducted for 4 years from 2006 to 2010 in Ludhiana, Punjab, India. Common weeds included burclover (Medicago arabica), common lambsquarters (Chenopodium album), littleseed canarygrass (Phalaris minor), purple nutsedge (Cyperus rotundus), scarlet pimpernel (Anagallis arvensis), swinecress (Coronopus didymus), and toothed dock (Rumex dentatus). Results suggested that atrazine applied alone was not very effective and resulted in 0% to 78% control depending on the weed species being investigated at 30 days after treatment (DAT). Straw mulch applied alone at any rate provided ≥90% control of toothed dock, but control of other weed species was variable. A combination of atrazine and straw mulch at any rate usually resulted in >90% weed control at 30 DAT, except for swinecress and purple nutsedge. This treatment combination also resulted in weed density as low as 0 plant/m2 for common lambsquarters, scarlet pimpernel, and toothed dock. Potato tuber weight and yield was significantly higher in all treatments compared with untreated control without difference among them. It is concluded that a combination of straw mulch and atrazine can provide effective weed control in potato.