Genetic diversity and population structure of Pisum sativum accessions for marker-trait association of lipid content

Ahmad, Sajjad; Kaur, Simerjeet; Lamb-Palmer, Neil Dylan; Lefsrud, Mark; Singh, Jaswinder

doi:10.1016/j.cj.2015.03.005

Cited by 30 publications

(23 citation statements)

References 31 publications

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“…The expected heterozygosity ( H e ) ranged from 0.212 (Elatius 3) to 0.477 (Nain Ordinaire), with a mean of 0.362. The PIC varied from 0.184 (Elatius 3) to 0.416 (Nain Ordinaire), with a mean of 0.321, indicating that the varieties comprised a considerable level of variability, which was higher than the variability level recorded in the pea germplasm by Ahmad et al [2]. This result reflects that the Nain Ordinaire accession has the highest level of genetic variability, but the Elatius 3 accession has the lowest variability level.…”

Section: Resultsmentioning

confidence: 67%

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

El‐Esawi

Al-Ghamdi

Ali

et al. 2018

IJMS

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Pisum sativum L. (field pea) is a crop of a high nutritional value and seed oil content. The characterization of pea germplasm is important to improve yield and quality. This study aimed at using fatty acid profiling and amplified fragment length polymorphism (AFLP) markers to evaluate the variation and relationships of 25 accessions of French pea. It also aimed to conduct a marker-trait associations analysis using the crude oil content as the target trait for this analysis, and to investigate whether 5-aminolevulinic acid (ALA) could enhance salt tolerance in the pea germplasm. The percentage of crude oil of the 25 pea genotypes varied from 2.6 to 3.5%, with a mean of 3.04%. Major fatty acids in all of the accessions were linoleic acid. Moreover, the 12 AFLP markers used were polymorphic. The cluster analysis based on fatty acids data or AFLP data divided the 25 pea germplasm into two main clusters. The gene diversity of the AFLP markers varied from 0.21 to 0.58, with a mean of 0.41. Polymorphic information content (PIC) of pea germplasm varied from 0.184 to 0.416 with a mean of 0.321, and their expected heterozygosity (He) varied from 0.212 to 0.477 with a mean of 0.362. The AFLP results revealed that the Nain Ordinaire cultivar has the highest level of genetic variability, whereas Elatius 3 has the lowest level. Three AFLP markers (E-AAC/M-CAA, E-AAC/M-CAC, and E-ACA/M-CAG) were significantly associated with the crude oil content trait. The response of the Nain Ordinaire and Elatius 3 cultivars to high salinity stress was studied. High salinity (150 mM NaCl) slightly reduced the photosynthetic pigments contents in Nain Ordinaire leaves at a non-significant level, however, the pigments contents in the Elatius 3 leaves were significantly reduced by high salinity. Antioxidant enzymes (APX—ascorbate peroxidase; CAT—catalase; and POD—peroxidase) activities were significantly induced in the Nain Ordinaire cultivar, but non-significantly induced in Elatius 3 by high salinity. Priming the salt-stressed Nain Ordinaire and Elatius 3 plants with ALA significantly enhanced the pigments biosynthesis, antioxidant enzymes activities, and stress-related genes expression, as compared to the plants stressed with salt alone. In conclusion, this study is amongst the first investigations that conducted marker-trait associations in pea, and revealed a sort of correlation between the diversity level and salt tolerance.

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Section: Resultsmentioning

confidence: 67%

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

El‐Esawi

Al-Ghamdi

Ali

et al. 2018

IJMS

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“…In pea, association and linkage mapping has been employed to uncover the genetic bases of several traits including agronomic and seed quality traits [30,35], disease resistance [32,36], seed mineral concentrations [37], seed lipid content [38], salinity tolerance [31], and frost tolerance [33]. Despite its importance, only limited studies have been carried out to identify genomic regions associated with pea stress tolerance [28].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea

Tafesse

Gali

Lachagari

et al. 2020

IJMS

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Environmental stress hampers pea productivity. To understand the genetic basis of heat resistance, a genome-wide association study (GWAS) was conducted on six stress responsive traits of physiological and agronomic importance in pea, with an objective to identify the genetic loci associated with these traits. One hundred and thirty-five genetically diverse pea accessions from major pea growing areas of the world were phenotyped in field trials across five environments, under generally ambient (control) and heat stress conditions. Statistical analysis of phenotype indicated significant effects of genotype (G), environment (E), and G × E interaction for all traits. A total of 16,877 known high-quality SNPs were used for association analysis to determine marker-trait associations (MTA). We identified 32 MTAs that were consistent in at least three environments for association with the traits of stress resistance: six for chlorophyll concentration measured by a soil plant analysis development meter; two each for photochemical reflectance index and canopy temperature; seven for reproductive stem length; six for internode length; and nine for pod number. Forty-eight candidate genes were identified within 15 kb distance of these markers. The identified markers and candidate genes have potential for marker-assisted selection towards the development of heat resistant pea cultivars.

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“…Molecular markers can be used effectively to study genetic diversity in crops (Ahmad et al, 2015). For the analysis of P. sativum var.…”

Section: Introductionmentioning

confidence: 99%

Forage pea (Pisumsativum var. arvense L.) landraces reveal morphological andgenetic diversities

Demirkol¹,

Yılmaz²

2019

Turk J Bot

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The objective of this study was to assess morphological and genetic diversities of 48 forage pea landraces collected from different locations at different altitudes in Turkey. Morphological, quality, and yield features were determined for the landraces and three control cultivars in three subsequent years. Genetic diversities of the landraces and cultivars were also monitored using microsatellite (SSR) markers. Our results revealed that the features of landraces are significantly different. The hay weights and the relative feed values were found to be significantly affected by altitude, with the landraces generally showing significantly higher hay weight and relative feed values at lower altitudes (P < 0.05). At the genetic level, 32 SSR primers led to distinct placement of one of the samples into a different clade of the dendrogram, showing that it is genetically different from the other 47 samples. This genetically different landrace had the highest forage value, suggesting that it shows higher prime forage features than the cultivars and the other landraces. Moreover, altitude and generally flower color were found to be important factors affecting the genetics of the landraces, as the landraces having white flowers or collected at similar altitudes were clustered well in the dendrogram. The results of this study reveal that the morphological and genetic diversities of forage pea landraces collected from different locations at different altitudes show variations. Such information could be used to develop forage pea landraces with improved characters that can be used in hay management.

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Genetic diversity and population structure of Pisum sativum accessions for marker-trait association of lipid content

Cited by 30 publications

References 31 publications

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea

Forage pea (Pisumsativum var. arvense L.) landraces reveal morphological andgenetic diversities

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