Coffee is one of the most consumed beverages in the world and is the second largest traded commodity after petroleum. Due to the great demand of this product, large amounts of residues are generated in the coffee industry, which are toxic and represent serious environmental problems. Coffee silverskin and spent coffee grounds are the main coffee industry residues, obtained during the beans roasting, and the process to prepare "instant coffee", respectively. Recently, some attempts have been made to use these residues for energy or value-added compounds production, as strategies to reduce their toxicity levels, while adding value to them. The present article provides an overview regarding coffee and its main industrial residues. In a first part, the composition of beans and their processing, as well as data about the coffee world production and exportation, are presented. In the sequence, the characteristics, chemical composition, and application of the main coffee industry residues are reviewed. Based on these data, it was concluded that coffee may be considered as one of the most valuable primary products in world trade, crucial to the economies and politics of many developing countries since its cultivation, processing, trading, transportation, and marketing provide employment for millions of people. As a consequence of this big market, the reuse of the main coffee industry residues is of large importance from environmental and economical viewpoints.
Interest in the development of bioprocesses for the production or extraction of bioactive compounds from natural sources has increased in recent years due to the potential applications of these compounds in food, chemical, and pharmaceutical industries. In this context, solid-state fermentation (SSF) has received great attention because this bioprocess has potential to successfully convert inexpensive agro-industrial residues, as well as plants, in a great variety of valuable compounds, including bioactive phenolic compounds. The aim of this review, after presenting general aspects about bioactive compounds and SSF systems, is to focus on the production and extraction of bioactive phenolic compounds from natural sources by SSF. The characteristics of SSF systems and variables that affect the product formation by this process, as well as the variety of substrates and microorganisms that can be used in SSF for the production of bioactive phenolic compounds are reviewed and discussed.
This study evaluated the efficacy of different solvents (methanol, ethanol, acetone, hexane, ethyl acetate, water, methanol:water mixtures, ethanol:water mixtures, and acetone:water mixtures) for extracting antioxidant phenolic compounds from brewer's spent grains (BSGs). The extracts were characterized regarding the contents of total phenols, flavonoids, proteins and reducing sugars. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and the ferric reducing antioxidant power (FRAP) assay. The solvents had different efficiencies for extraction of antioxidant phenolic compounds. All the produced extracts showed antioxidant activity, but the extract produced with 60% v/v acetone had the most elevated content of total phenols and antioxidant potential by the two methods. BSG was demonstrated to be a valuable source of antioxidant phenolic compounds, and solid-to-liquid extraction using 60% v/v acetone was a low cost and quite efficient method to recover these value-added compounds.
The extraction of antioxidant phenolic compounds from spent coffee grounds (SCG) was studied. Extraction experiments were carried out by the conventional solid-liquid method, using methanol as solvent at different concentrations (20-100%), solvent/solid ratios (10-40 ml/g SCG), and extraction times (30-90 min), and the influence of these operational variables on the content of total phenolic compounds and antioxidant activity of the produced extracts was evaluated. Flavonoids, chlorogenic acid, and protocatechuic acid were found in all the produced extracts and were also quantified. A strong influence (p < 0.05) of the variables on the extraction results was verified, and the conditions able to maximize each response (contents of total phenolic compounds, flavonoids, chlorogenic acid and protocatechuic acid, and antioxidant activity) were established. Extraction using 60% methanol in a solvent/solid ratio of 40 ml/g SCG, during 90 min, was the most suitable condition to produce a extract with high content of phenolic compounds (16 mg gallic acid equivalents/g SCG) and high antioxidant activity (FRAP of 0.10 mM Fe(II)/g), simultaneously. These findings are of interest since antioxidant phenolic compounds have an outstanding role in health area, and wide applications in food and pharmaceutical products.
This study provides a more accurate comparison between the antibiotic susceptibilities of planktonic versus biofilm populations, because the cell densities in the two populations were similar and because we measured the concentration required to inhibit bacterial metabolism rather than to eradicate the entire bacterial population. While the biofilm phenotype is highly resistant to antibiotics that target cell wall synthesis, it is fairly susceptible to antibiotics that target RNA and protein synthesis.
The emergence of new infections and increase of bacteria drug-resistance rise up the urgent need for the development of new antibacterial agents from natural sources. This study was designed to evaluate the antibacterial activity of the crude methanolic extract (CME) and fractions [hexane (H), dichloromethane (DCM), ethyl acetate (EA) and ethanol (Et)] obtained from Larrea tridentata (Sessé & Moc. Ex DC.) Coville leaves. The antibacterial activity was determined by the agar diffusion method against six strains of Gram-positive and Gram-negative bacteria. The micro-dilution method was applied for the determination of the minimal inhibitory concentration (MIC) of selected bacteria strains. HPLC analyses of tested samples were also carried out. The antibacterial activity of the samples was more effective inhibiting the growth of Gram-positive bacteria comparing with Gram-negative bacteria, mainly for the CME, DCM and EA fractions. EA fraction showed the highest antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from secretion; with a MIC value (31.3 g/mL) lower than the reference antibiotic tetracycline (64 g/mL). Low MIC values (62.5 g/mL) were also obtained for the CME and DCM fraction. CME and EA fraction presented the highest concentrations of quercetin, kaempferol and nordihydroguaiaretic acid. These compounds have important biological activities and could be responsible for at least part of the antibacterial activity of the CME, DCM and EA fractions. EA fraction from L. tridentata leaves was the most efficient to inhibit the growth of the bacterial strain methicillin-resistant S. aureus, which represents an important step for the search and development of a new antibacterial agent.
Low concentrations of antibiotics can inhibit microbial adherence to medical device surfaces. However, little is known about the changes that occur in the physiology of bacteria within biofilms formed in the presence of subinhibitory (sub-MIC) concentrations of antibiotics. In this study, the densities and matrix compositions of biofilms formed by two coagulase-negative Staphylococcus species in the absence and in the presence of sub-MIC concentrations of dicloxacillin were evaluated. Biofilms formed in the presence of sub-MIC concentrations of dicloxacillin contained less biomass, and there were notable changes in the composition of the biofilm matrix. Changes in the spatial structure were also verified by confocal scanning laser microscopy, indicating that biofilms grown in the presence of sub-MIC concentrations of dicloxicilln had a lower cell density. Physiological alterations in the bacteria within biofilms grown in the presence of subinhibitory concentrations of the antibiotic were also evaluated. The results showed that there were differences in bacterial surface characteristics when cultures were grown in the presence of sub-MIC concentrations of dicloxacillin, including decreased hydrophobicity and decreased expression of the exopolysaccharide poly-N-acetylglucosamine. The elemental composition of the cell surface was also analyzed, and whereas in Staphylococcus epidermidis there were decreases in the oxygen and nitrogen contents, in Staphylococcus haemolyticus there were increases in these two parameters. Additionally, increases in resistance to several antibiotics were observed for the cells within biofilms formed in the presence of dicloxacillin.
Methanol, ethanol, and acetone at four different concentrations (90%, 70%, 50%, and 30% v/v) were used for extraction of phytoestrogens (nordihydroguaiaretic acid (NDGA), kaempferol, and quercetin) from Larrea tridentata leaves. Besides the phytoestrogens extraction, the antioxidant potential, and the contents of total phenols, flavonoids, and protein in the produced extracts were also determined. The solvent and concentration used for extraction strongly affected the phytoestrogens recovery. The highest NDGA, quercetin, and kaempferol contents (46.96 ± 3.39, 10.46 ± 1.01, and 87.00 ± 6.43 mg/g DW plant, respectively) were recovered using 90% (v/v) methanol. All the produced extracts showed antioxidant capacity, but those obtained using 70% and 90% (v/v) methanol had significantly higher (p < 0.05) FRAP (ferric reducing antioxidant power) values (2.55 ± 0.09 and 2.73 ± 0.11 mM FE(II)/g DW plant, respectively) than the remaining ones. Extract produced by using 90% (v/v) methanol contained also the highest contents of total flavonoids (19.29 ± 0.79 mg QE/g DW plant) and protein (131.84 ± 6.23 mg/g DW plant), and elevated total phenols concentration (263.60 ± 25.78 mg GAE/g DW plant).
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