Burns patients are at high danger of nosocomial disease, and Pseudomonas aeruginosa is one of the mainly common causes of wound and systemic infection resulting in significant morbidity and mortality in burns patients. The appearance of multidrug resistant strains is up surging leading to problematical control. The aim of this study was to isolate and identify MDR-P. aeruginosa from the burn patients held in the burn unit and study the antibacterial activity and mechanism of Nickle Nanoparticles solution on bacterial isolated and evaluate by molecular and pathological techniques. This study was carried out on the Burn patients in Tikrit teaching hospital in Tikrit city / Iraq from January, 2017 to June, 2017. The scientific samples were collected using sterile cotton swabs from 60 patients with burn infection. P. aeruginosa that were recognized by cultural characteristics, Gram stain, and biochemical reactions. The results of the laboratory cultural of 60 cotton swabs used showed to isolated 35(58.3%) P. aeruginosa isolated and all bacterial isolates were resistant to Doxycycline hydrochloride, Penicillin, Cotrimoxazole, Ciprofloxacin, Cephalosporin and Penicillin. The study showed that the Nickel Ferrite (NiFe 2 O 4 ) used to inhibit the growth of bacterial isolated by using different concentrations the MBC killer concentration was 256 μg / L and the lowest inhibitory concentration to P. aeruginosa was MIC 32 microgram / L). Molecular studies included the observation of the most important molecular changes at the level of DNA prior to and treatment with nanoparticles. Many variations were observed on the studied bacterial isolated. Variations include the appearance and disappearance of DNA and its different numbers when treated with nanoparticles. As for the results of the histopathological, it was found that the injury of mice with three antibiotic resistance isolates emerged after five days and the symptoms were heat, redness and swelling of the skin and the release of yellow and green purulent secretions from the place of injury. Which were treated with antimicrobial and nanoparticles together was faster than the time of the healing of nanoparticles treated only.
The bacteria have been noted as the main cause of late wound healing. The greatest common pathogen causing the wound contaminations is Staphylococcus aureus. The current study was carried out to isolate and diagnose the staphylococcus aureus which causes of open wound inflammation after surgery in mice process and to study the effect of nickle nanoparticles solution on bacterial isolated and evaluating the molecular and pathological techniques. The study included the collection of 60 cotton swabs from the Office of the Consultant of the Faculty of Veterinary Medicine – Tikrit University and from the external veterinary clinics (from November 2018 to March 2019) from the areas of contaminated wounds or inflamed after surgery. The results of the laboratory cultural of 60 cotton swabs used showed to isolated 50(83.3%) Staphylococcus aureus isolated. And all bacterial isolates were resistant to Doxycycline hydrochloride, Penicillin, CO-Trimoxazole, Ciprofloxacin, Cephalosporin and Penicillin. The study showed that the NFNPS used to inhibit the growth of bacterial isolated by using different concentrations the MBC killer concentration was 256μg / L and the lowest inhibitory concentration to Staphylococcus aureus was MIC 64 microgram / L). Molecular studies included the observation of the most important molecular changes at the level of DNA prior to and treatment with nanoparticles. Many variations were observed on the studied bacterial isolated Including the appearance and disappearance of DNA and its different numbers when treated with nanoparticles.As for the results of the histopathological, it was found that the injury of mice with Staphylococcus aureus antibiotic resistance emerged after about five days and the symptoms were heat, redness and swelling of the skin and the release of yellow and green purulent secretions from the place of injury. When treated mice infected with nanoparticles and antibiotics together the time of the healing was faster than the time of the healing of nanoparticles treated only.
An overdose of tramadol is a frequent reason for hepatic and renal toxicity and possible death.Hence, this study aimed at investigating the impact of tramadol on serum liver enzyme, oxidative stressm and som antioxidant markers in male rabitts. Methods: A total of 20 growing rabbits (7.5 weeks old) reared under high ambient temperature were divided into two equal groups, 10 rabbits each. The first group control administered with normal saline and the second group tramadol-treated rats (50 mg/kg b.w. orally) for 30. Blood samples were withdrawn to measure serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Glutathione (GSH), malondialdehyde (MDA) levels and catalase (CAT), activities were assayed. Results: In tramadol-treated group, significant increases in aspartate aminotransferases, and alanin aminotransferases activities, malondialdehyde (MDA) levels and significant decreases in catalase (CAT), glutathione activities levels were determined compared to the control group. Conclusion: tramadol administration produces noticeable biochemical changes in a dosedependent manner associated with increased liver enzyme and oxidative stress markers and decreased antioxidative activity.
Aspirin (Acetylsalicylic acid) is widely used in human and veterinary as anti-inflammatory cardiovascular prophylaxis, anti-clotting agent and to decrease cancer risk. However, it is frequently mis- or over-consumed to prevent pain . Many hazards linked with its use have been recorded, but there is a lack of data on its effects on androgenic studies, testicular histology, kidney histology, liver histology, and reproductions. We tried to gather information on its adverse toxicity effects on male rat reproductive in this research. According to research, aspirin has a fatal effect on sperm production and reproductive hormones, as well as lowering testicular weight and altering blood profile. Because aspirin is a prostaglandin inhibitor, it may have an effect on the male reproductive system. Aspirin's detrimental toxic character is further revealed by liver damage.
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