The results are reported of a collaborative study in which five meat products containing different known levels of one of five different commercial soya ingredients, together with a blind duplicate and a blank, were analysed for soya protein by 26 laboratories in 10 European countries. Two techniques were tested: the sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). method of Armstrong et al. (J. Food Technol. 1982, 17, 327-337) and the enzyme-linked immunosorbent assay (ELISA) method of Hitchcock et al. (J. Sci. Food Agric. 1981, 32, 157-165). It was concluded that both methods give zero blanks and similar interlaboratory variances; SDS-PAGE gives more repeatable intralaboratory data, while ELISA gives more accurate determinations. The results reflect significant progress to an interim stage of methodology development; both methods are useful, but require further refinements to make them generally acceptable for control purposes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.