Objective: The purpose of this study was to determine whether or not individuals with iron deficiency anemia and an underlying inflammatory condition had a connection between serum ferritin as an acute phase reactant and C - reactive protein. Study Design: Cross-sectional/Prospective study Place and Duration: Liaquat university of Medical and health sciences jamshoro Hyderabad. January 2022 to December 2022 Methods: This research comprised 136 individuals who were suffering from iron deficiency anemia. After getting informed written consent detailed demographics of enrolled cases were recorded. Using an automated hematology analyzer (Mindray BC-5000), the parameters of the blood were analyzed. Serum ferritin levels were used to classify each patient into one of three categories. Serum ferritin levels were divided into three groups: Group I (serum ferritin <10 μg/L), Group II (11–150 μg/L), and Group III (>150 μg/L).SPSS version 23.0 was used to analyze the data. Statistical analysis was conducted using Pearson's correlation tests. Results: In current study patients mean age was 31.13±6.75 years and had mean BMI 24.6±3.52 kg/m2. There were majority 74 (54.4%) males and 62 (45.6%) females in this study. Majority of the patients 81 (59.6%) were had low ferritin <10 μg/L, followed by normal ferritin 11–150 μg/L in 45 (33.1%) cases and 10 (7.4%) cases had high ferritin >150 μg/L. We found strong co-relation of C reactive protein and high level of serum ferritin with p value <0.002. Serum ferritin levels were negatively correlated with hemoglobin. Conclusion: We concluded in this study that levels of serum ferritin was positively associated with C-reactive protein (CRP). In patients with underlying deficiency of iron, secondary inflammation may increase the level of ferritin in serum. Keywords: Ferritin, Haemoglobin, Iron Deficiency Anaemia, C-Reactive Protein

Objective: The purpose of this research was to better understand the factors that lead to pre-donation deferral at a tertiary care hospital's Blood bank. Understanding the factors that lead donors to decline can inform more effective selection criteria. Study Design: Cross-sectional/Descriptive study Place and Duration: Diagnostic and research laboratory Liaquat university of Medical and Health Sciences Jamshoro Hyderabad. January 2022-December 2022 Methods: This study comprised of 2200 donors at blood bank of LUMHS. The UDHQ included 25 questions to assess donors' backgrounds in regards to things like injections, medicines, time since last donation, tattoos, general health, dental work, sexual relationships, malaria/T.B., surgery, jaundice, vaccination, positive viral markers, etc. Haemoglobin (Hemacue 50/Diaspect Hb with daily quality control), Heart rate/rhythm, Blood pressure, Body temperature, Weight/Height, and Jaundice were all part of the physical exam. Results: There were majority 1578 (71.7%) males and 622 (28.3%) female donors among all cases. Donors mean age was 26.12±9.88 years and mean weight was 61.3±8.49 kg. 1435 (65.2%) cases were from urban areas. Among all, 1650 (75%) donors were accepted and 550 (25%) donors were deferral. Most common cause of deferral was low hemoglobin followed by anemia, HCV, HBV, inappropriate pulse rate, low BP, jaundice, active infection, malaria, syphilis, allergy, aspirin intake, drugs, last donation, skin/eye colour and sexual relationship. There were 1870 (85%) cases of replacement donors among all cases. Conclusion: The selection of healthy blood and the reduction of donor injury will be made possible by a thorough pre-donation screening interview and physical examination. Our setup has a somewhat higher blood donor deferral rate because to stringent donor sector requirements and extra attention paid to donor selection processes. The increased replacement donor deferral rate is a result of refusals for past injection history and poor hemoglobin levels. Keywords: Donors, Deferrals, Hemoglobin, Infection, HCV

Objective: The purpose of this research is to examine the relationship between FLT3 mutation and hematological indices and clinical correlates in patients with severe myelogenous leukemia & acute lymphoblastic leukemia. Study Design: Descriptive Study. Place and Duration: Diagnostic and research Laboratory Liaquat university of Medical and health sciences jamshoro Hyderabad. January 2021 to December 2021. Methods: Total 79 cases of acute leukemia were included in this study. Patients provided written consent for detailed demographics and for self-structure questionnaire. All the data was obtained from laboratory records. The patients' clinico-hematologic and immunophenotypic characteristics were recorded, and the FLT3 mutation was analyzed molecularly. SPSS 24.0 was used to analyze all data. Results: Majority of the patients 44 (55.7%) were males and 35 (44.3%) cases were females. The included patients had mean age 37.21±14.59 years and had mean BMI 23.6±4.18 kg/m2. We found that 48 (60.8%) cases had acute myeloid leukemia (AML) and 31 (39.2%) cases had acute lymphoblastic leukemia (ALL). Frequency of FLT 3 mutations in AML patients was 6 (12.5%) and in ALL patients was 1 (3.2%) after polymerase chain reaction. WBC was the most statistically significant hematological and clinical result connected with FLT3 mutation.

Objective: The goal was to determine if antiplatelet medication was effective at preventing preeclampsia in high risk patients as well as its negative effects. Study Design: Descriptive case series Place and Duration: Gynaecology and obstetrics department LUMHS Jamshoro. Jan 2022-Dec 2022 Methods: Total 135 pregnant females of age 20-40 years were included in this study. The included patients were all high risk pregnancies with parity 5, gestational age 12 weeks, and hospital prenatal checkups. Aspirin (an antiplatelet medication) 120 mg/day was administered to all the females. They were monitored in the OPD up until the 36th week of pregnancy. Preeclampsia was diagnosed in females who had BP > 140/90 mmHg and proteinuria > 300 mg using the urine dipstick technique. All data were examined using SPSS 24.0. Results: There were 95 (70.4%) cases had age <30 years and 40 (29.6%) females had age >30years. Mean parity of the females was 2.3±5.11. Mean BMI was 25.02±6.35 kg/m2. There were 60 (44.4%) females were educated and 55 (40.75) females were from urban areas. History or preeclampsia found in 7 (5.2%) cases. There were 28 (20.7%) females were obese, chronic hypertension found in 9 (6.75) cases, gestational hypertension in 92 (68.1%) cases and gestational diabetes in 22 (16.3%) cases. Frequency of preeclampsia was found in 19 (14.1%) cases. Other complications were low platelet, acid peptic disease and Antepartum haemorrhage. Conclusion: We observed that the occurrence of preeclampsia was extremely low among high-risk females who had 120mg of Aspirin (antiplatelet medication) in the first trimester, but that the negative effects of antiplatelet therapy increased with increasing doses. Keywords: Pregnant females, Gestational Hypertension, Antiplatelet Therapy, Preecplampsia

Objective: This study was designed to determine the pattern of anaemia among HIV positive patients taking HAART. Study Design: Cross-sectional study. Place and Duration: Diagnostic and research Laboratory Liaquat university of Medical and health sciences Jamshoro, Hyderabad. January 2022-December 2022. Methods: Total 250 HIV patients (aged 18 years and above) of both genders taking HAART for at least the past six months. After taking written informed consent, data was recorded with the help of a structured questionnaire that contained questions regarding basic bio-data and medical history, along with the interpretation of general physical and systemic examination. A blood complete picture was done to evaluate baseline haematological parameters. Data were analyzed using Statistical Package for Social Sciences (SPSS) version 22.0. Results: The mean age of the patients was 32.09 (±SD12.165). Most of the individuals, i.e., 160 (64%), were males, and the number of females was 90 (36%). The mean viral load was 2674.60. Anaemia was present in 210 (84%) patients and 40 (16%)non-anemic with standard parameters. Among anaemic patients, 130 52% had mild disease, moderate anaemia was found in 105 (42%), and only a few (n=15) 6% had severe anaemia.