This study was focused to investigate comparative hematological changes in Schizothorax richardsonii in relation to reproductive activities. The values of Red blood cells (RBC) were significantly higher in both the sexes during the immature stage as compared with maturity phase. Female brooders reflect higher count of WBC in females during both the phases. However, no significant difference was observed in White blood cell (WBC) count during immature and mature stage. Mean corpuscular volume (MCV) and Mean corpuscular hemoglobin (MCH) showed significant difference (P<0.05) in both the phases of maturity. The values of MCH were at higher side in males during the maturity phase. Hematological values of Hematocrit (Hct), Total erythrocyte count (TEC)/Red Blood Corpuscles and Mean corpuscular hemoglobin concentration (MCHC) were significantly higher in postspawning phase than the pre-spawning phase. MCHC values of males are in higher side during pre-spawning phase than the post-spawning period, while higher values of MCHC were observed in females during post-spawning phase. Present study reveals that MCV is at higher side in female during pre spawning phase expressing that it is due to lower Hct in females as MCV reflects the volume occupied by a single RBC. Present findings reflect the major factors responsible for the variation in hematological parameters during reproduction of fish.
Labeo gonius, an indigenous medium carp of the Cyprinidae family, is a potential species for polyculture with exotic carps in foothills and mid-latitudes. Apart from the plain and Tarai region, it is widely distributed all along foothill regions of Himalayan region in India with a great food value and fair market demand. An attempt was made to establish a breeding protocol and to assess the Gonadosomatic Index (GSI) and Fecundity of this hill aquaculture potent fish species. A total of 41 brooders were selected and injected intramuscularly with Ovatide @ 0.3, 0.5, and 0.7 ml/kg bw in females and 0.1, 0.2, and 0.3 ml/kg bw in males, and absolute fecundity were recorded between 72265 to 184322 ova with an average of 111330.8625±53449 ova per fish. Water temperature between 18-22 C was observed optimum for the egg incubation (48 h) with a higher hatching rate (82%) and better recovery of hatchlings. The spawning fecundity was observed as 203478 eggs/kg bw. GSI was observed by dissection of selected brooders which ranged from 3.324 ± 0.143 to 14.115 ± 1.214 which increased gradually from April to July and decreased in August in captive conditions. The highest value of GSI was recorded in July (14.115 ± 1.214) while the lowest was observed in January (3.324 ± 0.143). The single peak of GSI during July indicates the spawning season of L gonius in July-August. The present findings highlight the reproductive potential of L. gonius in coldwater conditions which enable the breeding and seed production aquaculture of this indigenous fish species in hills.
The present paper investigated the Physico-chemical parameters of the lotic water body Shipra, including its two sites (Site 1- Kainchi, Site 2- Garampani) for six months (October 2020-March 2021) of Nainital District in Uttarakhand. Monthly variations in the physical and chemical parameters at site-1 such as-- Atmospheric Temperature 14.1-26 C, Water Temperature 11-15 C, pH 7-8.3, Total Dissolved Solids 76-92 ppm, Transparency 36- 78 cm, Dissolved oxygen 6.04-9.26 mg/l, Phosphate 0.25-0.43 mg/l and Nitrate 0.34-1.63 mg/l and similarly for site-2 Atmospheric Temperature 9-24 C, Water Temperature 10-18 C, pH 8.2-8.7, Total Dissolved Solids 111-132 ppm, Transparency 31-58 cm, Dissolved oxygen 8.05-9.26 mg/l, Phosphate 0.11-0.32 mg/l and Nitrate 0.32-0.55 mg/l were observed. All the parameters of site-1 and site-2 were within the acceptable limit except for the pH value at site-2.
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