The present study was conducted to investigate the influence of dietary supplementation of root powder of Ashwagandha (Withania somnifera), flower powder of Butea monosperma, seed powder of Mucuna pruriens and dried powder of Jaiphal (Myristica fragrans) on gonadal maturity and milt quality in male brood stock of commercially important indigenous coldwater fish, snow trout, Schizothorax richardsonii in captive condition. Experimental fish of 3 years age were reared in FRP tanks (n=6) of 1200 L capacity with stocking density of n=60 in each tank and fed with control (CD) and experimental (TD) diets in duplicate at the feeding rate of 3-5% of their body weight twice a day. Gonadosomatic index (GSI) and Hepatosomatic index (HSI) were recorded for annual breeding cycle coupled with breeding indices and sperm density in breeding season along with regular monitoring of water quality parameters. In males, the GSI values ranges from 0.49 to 7.92 and 0.85 to 11.83 in control and treated groups, respectively. Two peaks of GSI values reflects the two breeding season of the species during the month of March and September. Similarly, HSI values vary from 0.41% to 0.52% and 0.44% to 0.58% in control and treated groups, respectively. In captive condition, 92% specimen were observed mature for spawning in treated group, while only 32% males were ready to oozing the milt in control stock. The fertilization rate (September 2019) was observed as 94.2% in treated group, while it was 86% in control group.The mean value of sperm count per ml. of milt (September 2019) was 3.64±0.66×10 8 /ml in control group and 4.44±0.64×10 8 /ml in treated group. The study revealed that 1.0 % supplementation of blend of herbs in the diet is beneficial for gonadal maturity in males and quality milt production of Schizothorax richardsonii in captivity under coldwater condition.
This study was focused to investigate comparative hematological changes in Schizothorax richardsonii in relation to reproductive activities. The values of Red blood cells (RBC) were significantly higher in both the sexes during the immature stage as compared with maturity phase. Female brooders reflect higher count of WBC in females during both the phases. However, no significant difference was observed in White blood cell (WBC) count during immature and mature stage. Mean corpuscular volume (MCV) and Mean corpuscular hemoglobin (MCH) showed significant difference (P<0.05) in both the phases of maturity. The values of MCH were at higher side in males during the maturity phase. Hematological values of Hematocrit (Hct), Total erythrocyte count (TEC)/Red Blood Corpuscles and Mean corpuscular hemoglobin concentration (MCHC) were significantly higher in postspawning phase than the pre-spawning phase. MCHC values of males are in higher side during pre-spawning phase than the post-spawning period, while higher values of MCHC were observed in females during post-spawning phase. Present study reveals that MCV is at higher side in female during pre spawning phase expressing that it is due to lower Hct in females as MCV reflects the volume occupied by a single RBC. Present findings reflect the major factors responsible for the variation in hematological parameters during reproduction of fish.
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