Background: MicroRNAs (miRNAs) play a significant role in shaping immune response. The expression profile of miRNAs, however, has been found to be affected by the presence of tumor, but whether the incidence of non-small cell lung cancer (NSCLC) alters the expression profile of miRNAs has not been studied. Objective: The current study aims to investigate the expression of miRNAs in the peripheral blood mononuclear cells (PBMCs) from patients with NSCLC as well as to analyze the numbers of helper CD4 + and cytotoxic CD8 + T lymphocytes in PBMCs of the same patients as compared to healthy controls. Methods: PBMCs were prepared from blood harvested from early-diagnosed patients with NSCLC (n=10). The gene expression of miRNAs in PBMCs was measured using microarray and the phenotypic analysis of helper CD4 + T-cells and cytotoxic CD8 + T-cells in PBMCs was determined by flow cytometry. Results: A significant upregulation of miRNA-21 (2-fold) and downregulation of miRNA-155 (-1.5fold) was observed in the PBMCs of NSCLC patients as compared to healthy controls. The relative and absolute numbers of CD4 + and CD8 + T-cells were significantly elevated in the PBMCs of NSCLC patients. Conclusion: Our results indicate that both expression levels of miRNA-21 and miRNA-155 in PBMCs and the numbers of CD4 + and CD8 + T-cells are altered by the presence of NSCLC.
Background: Lung cancer is the leading cause of cancer-related death worldwide with a substantially low survival rate. MiRNAs have been shown to regulate self-renewal and differentiation properties of CSCs, Here, MicroRNAs play a significant role in shaping immune response. Aims: the current study aims to analyze the numbers of helper CD4+ and cytotoxic CD8+ T lymphocytes, CD133+ and Epcam+ CSCs. also, measure the relative expression of miRNAs in Non-small cell lung cancer patients before and after treatment. Materials and Methods: The frequencies of Epcam+ and CD133+ cells and the numbers of helper CD4+ and cytotoxic CD8+ T lymphocytes were analyzed in the peripheral blood of NSCLC patients before (n= 10) and after (n= 10) chemotherapy using multiparametric flow cytometry. The gene expression of miRNAs was measured using microarray.Results: Early diagnosed patients before treatment showed high numbers of Epcam+ and CD113+ CSCs when compared to CTRL; where the numbers of these cells were decreased after treatment as compared to early diagnosed patients. A significant upregulation of miRNAs and downregulation of miRNAs were observed in the peripheral blood of NSCLC patients as compared to healthy control. The relative and absolute numbers of CD4+ and CD8+ T-cells were significantly elevated in the peripheral blood of NSCLC patients.
Conclusion:This study opens a new avenue to investigate the mechanism mediating the emergence of these cells on larger number of NSCLC patients at different treatment stages. We also discuss the role of microRNAs in therapeutic resistance and as biomarkers.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.