The intracellular lipids are reserved in LDs, and LDs also play key roles in collecting the intracellular toxic acids. [7] Furthermore, many crucial bioactivities cannot be achieved by single organelle, but be performed under the collaboration of multiple organelles. [8] For example, the lipid metabolism under innutritive conditions is accomplished via the cooperation of mitochondria, lysosomes, and LDs. The LDs are initially delivered into lysosomes and digested into fatty acids via autophagy. [9] The fatty acids are then transported into mitochondria and transformed to energy via β-oxidation. [10] Therefore, studying the interplay between multiple organelles is a crucial task to explore the mystery of life.Although the interactions between different organelles have been visualized via electron microscopy, [11] such observational method requires fixed cells to disrupt sample activity and is therefore not suitable for dynamic observation of living cells. In comparison, fluorescent probes are currently the desirable tools. Fluorescent imaging is an invasive analysis method, which can realize real-time, in situ, and dynamic visualization with negligible damage to biological samples. [12,13] Up to now, fluorescent probes to image single organelles have been widely reported. For example, cationic organic fluorescent probes were used to image mitochondria. Yu and co-workers have developed a phospholipid mimic probe with positive charge to visualize mitochondria in living cells and tissues with high fidelity. [14] Lysosomal probes were usually designed using alkalescence groups. Kim et al. have reported a two-photon ratiometric fluorescent probe for lysosomal pH, using secondary amines as the targeting group. [15] Neutral lipophilic fluorescent dyes can usually target the LDs. Tang's group has constructed a lipophilic fluorescent probe with aggregation-induced emission to image the LDs in living cells and liver tissues. [16] To reveal the interactions between organelles, simultaneous visualization of at least two organelles is necessary. Consequently, single fluorescent probes for separate visualization of two organelles have been developed very recently. [17] Han's group has presented a fluorescent probe to distinguish mitochondria and lysosomes in dual emission, and utilized the probe to visualize mitochondrial depolarization. [18] For example, Yang and co-workers have elegantly developed a fluorescentThe in-depth study of the interplay and cooperation between multiple organelles is an important biological task. Single fluorescent probes for separate visualization of multiple organelles is a desirable molecular tool, but the construction of such a probe is extremely difficult owing to the lack of valid strategies. In this work, utilizing the reversible cyclization reaction and intermolecular π stacking mechanism, a robust fluorescent probe is constructed to discriminatively illuminate lipid droplets (LDs), mitochondria, and lysosomes with blue, green, and red emission colors, respectively. Using the probe, the interp...