Intramolecular cyclization reaction enabling fluorescent probes for dual-color visualization of lysosomes and lipid droplets with prominent spectral shift

A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

“…Lipid droplets (LDs) are the main sites of lipid storage in eukaryotic cells, and they are mainly composed of neutral lipids (cholesteryl esters and triglycerides) and encapsulated in a phospholipid monolayer. 1,2 As a highly dynamic organelle, LDs are considered to play a non-negligible role in various physiological processes in the human body. 3 In addition to affecting vital activities, such as lipid metabolism and storage and signal transduction, they are also closely related to protein degradation, energy homeostasis, membrane protein expression, membrane transport, and cell migration, differentiation, proliferation and apoptosis.…”