The robot-assisted technique was associated with equivalent accuracy rate of pedicle screw implantation, fewer proximal facet joint violation, less intraoperative radiation exposure but longer surgical duration than freehand technique. Powerful evidence relies on more randomized controlled trials with high quality and larger sample size in the future.

Single-atom catalysts (SACs) have attracted much attention due to their outstanding catalytic performance in heterogeneous catalysis. Here, we report a template sacrificial method to fabricate an atomically dispersed Co catalyst; three kinds of silica templates with different microstructures (MCM-41, SBA-15, and FDU-12) were employed and the effect of pore structure of the templates on the dispersity of Co was investigated. The catalysts fabricated with different templates presented different Co dispersities, leading to distinguishing catalytic performance. The optimized Co 1 @NC-(SBA) catalyst with atomically dispersed Co displayed outstanding catalytic activity for the hydrodeoxygenation (HDO) of lignin-derived species as well as the hydrogenation of various nitroaromatics. The reaction mechanism of the HDO of vanillin was investigated by using density functional theory calculations as well.

BackgroundTongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect.Materials and methodsWe analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 μg/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 μg/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells.ResultsThe viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 μg/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells.ConclusionBased on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells.

Hexabromocyclododecanes (HBCDs) are raising concern because of their potential persistence, bioaccumulation, and toxicity. In this study, we investigated the concentrations, diastereoisomer- and enantiomer-specific profiles, and mass inventories of HBCDs in 90 surface soils from two e-waste recycling areas (Qingyuan, Guiyu) and from industrial areas in South China. The mean concentrations of total HBCDs in the surface soils ranged from 0.22 to 0.79 and from 0.31 to 9.99 ng/g dw for two surrounding e-waste recycling sites and industrial areas, respectively. The highest total HBCD concentration (284 ng/g dw) was found at the e-waste recycling site in Qingyuan, while total HBCD levels fell dramatically with increasing distance from the recycling site, suggesting that the e-waste recycling activities were an important source of HBCDs. The diastereoisomer profiles in 75 of the 90 soil samples differed from those of the commercial products. The mean enantiomeric fraction values for α-, β-, and γ-HBCDs in soils ranged from 0.503(0.010) to 0.507(0.003), 0.494(0.003) to 0.506(0.009), and 0.502(0.003) to 0.511(0.006), respectively, suggesting that there was no stereoselective transformation of the three diastereoisomers. The mass inventories of HBCDs gave preliminarily estimates of 3.42 kg and 1.84 tonnes for the e-waste recycling areas and industrial areas, respectively. It is notable that the diasteroisomer and enantiomer profiles of this study failed to distinguish definitely that the isomeric transformation occurred during the product processing or in the environmental matrix. Further laboratory studies on abiotic and biotic transformation are needed to clarify this issue.

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The rs12722 polymorphism in COL5A1 gene has been implicated in the etiology of musculoskeletal soft tissue injuries in several association studies with limited sample size and conflicting results. The purpose of the present systematic review and meta-analysis was to evaluate and synthesize the currently available data on the association between rs12722 and musculoskeletal soft tissue injuries. Five electronic databases including Pubmed, EMBASE, ISI Web of Science, CNKI and Wanfang were searched to identify relevant studies published before 15 May, 2017. Summary odds ratios (ORs) and corresponding 95% confidence intervals (95% CIs) were estimated using the RevMan 5.3 software. Nine studies comprising 1140 cases and 1410 healthy controls met the eligibility criteria. Recessive model was confirmed to be the optimum model (TT vs TC + CC). The results indicated that rs12722 SNP was significantly associated with musculoskeletal soft tissue injuries (OR 1.58, 95% CI 1.33, 1.89; P < 0.00001). When stratified by injury sites, modest but statistically significant association was found in Achilles tendon pathology (ATP), anterior cruciate ligament injuries (ACLI) and tennis elbow (TE). Subgroup-analysis by ethnicity suggested that TT genotype of rs12722 was associated with tendon and ligament injuries in Caucasians (OR 1.59, 95% CI 1.33, 1.90; P < 0.00001) but not in Asians (OR 1.46, 95% CI 0.46, 4.60; P = 0.52). Our findings indicated that rs12722 of COL5A1 was positively associated with tendon and ligament injuries, especially in Caucasian subjects. Individuals with TT genotype were predisposed to higher risk of ATP, ACLI and TE.

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