Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy

Wang, Jianfeng; Gao, Shutao; Wang, Shuyu; Xu, Zhenglin; Wei, Limin

doi:10.2147/ijn.s165699

Cited by 94 publications

(60 citation statements)

References 37 publications

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“…It has been reported that ZnONPs induced significant cytotoxicity with increased intracellular ROS and oxidative stress that led to apoptosis and autophagy in SKOV3 ovarian cancer cells [125]. ZnONPs have also been observed to induce toxicity by activating PINK1/Parkin-mediated mitophagy in CAL27 oral cancer cell lines [126]. In addition, some studies reported that ZnONPs exhibited preferential cytotoxicity to highly proliferative tumor cells through a lysosome-mediated zinc ion release mechanism that subsequently led to ROS-mediated cell death [124,127].…”

Section: Zno Nanoparticles-induced Toxicity and The Possible Role Of mentioning

confidence: 99%

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Chen

Liao

et al. 2020

IJMS

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Nanotechnology has rapidly promoted the development of a new generation of industrial and commercial products; however, it has also raised some concerns about human health and safety. To evaluate the toxicity of the great diversity of nanomaterials (NMs) in the traditional manner, a tremendous number of safety assessments and a very large number of animals would be required. For this reason, it is necessary to consider the use of alternative testing strategies or methods that reduce, refine, or replace (3Rs) the use of animals for assessing the toxicity of NMs. Autophagy is considered an early indicator of NM interactions with cells and has been recently recognized as an important form of cell death in nanoparticle-induced toxicity. Impairment of autophagy is related to the accelerated pathogenesis of diseases. By using mechanism-based high-throughput screening in vitro, we can predict the NMs that may lead to the generation of disease outcomes in vivo. Thus, a tiered testing strategy is suggested that includes a set of standardized assays in relevant human cell lines followed by critical validation studies carried out in animals or whole organism models such as C. elegans (Caenorhabditis elegans), zebrafish (Danio rerio), and Drosophila (Drosophila melanogaster)for improved screening of NM safety. A thorough understanding of the mechanisms by which NMs perturb biological systems, including autophagy induction, is critical for a more comprehensive elucidation of nanotoxicity. A more profound understanding of toxicity mechanisms will also facilitate the development of prevention and intervention policies against adverse outcomes induced by NMs. The development of a tiered testing strategy for NM hazard assessment not only promotes a more widespread adoption of non-rodent or 3R principles but also makes nanotoxicology testing more ethical, relevant, and cost- and time-efficient.

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Section: Zno Nanoparticles-induced Toxicity and The Possible Role Of mentioning

confidence: 99%

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Chen

Liao

et al. 2020

IJMS

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“…Recentemente, com o avanço da nanomedicina, tornou-se um importante componente utilizado para a produção de NP. A eficácia das moléculas de zinco contra células de carcinoma hepatocelular (HepG2), câncer de próstata e câncer de ovário tem sido relatada em vários estudos experimentais 21 .…”

Section: Resultsunclassified

“…As NP à base de metais, principalmente de zinco, apresentam significantes efeitos geradores de autofagia que facilitam e potencializam os efeitos causados pela ERO e, consequentemente, induzem com maior facilidade a morte de células cancerígenas, também causada pela flexibilidade das moléculas de zinco, o que as tornam ótimas moléculas carreadoras também 21,30,31 .…”

Section: Resultsunclassified

Utilização de Nanopartículas no Tratamento do Câncer: Aspectos Gerais, Mecanismos de Ação Antineoplásicos e Aplicabilidades Tumorais

Lopes¹,

Torres²

2020

Rev. Brasileira.De.Cancerologia

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Introdução: Os avanços tecnológicos das últimas décadas levaram ao desenvolvimento de abordagens terapêuticas na medicina em uma nova escala de aplicações moleculares nanométricas, permitindo o estabelecimento de novas terapias como a nanoterapia. O uso de nanopartículas implica inúmeras oportunidades e também grandes desafios para a aplicação humana, esse uso requer uma compreensão minunciosa da biocompatibilidade e da farmacodinâmica das moléculas. No câncer, as nanopartículas atuam principalmente na forma ativa, que se caracteriza pela utilização de ligantes ou anticorpos em suas superfícies onde se ligam a determinadas células, e, na passiva, onde as partículas se acumulam nos tecidos tumorais em razão das largas frenestrações dos endotélios tumorais. Objetivo: Abordar as principais propriedades das nanopartículas de proteína, metal, polímero e lipídio, suas estruturas e utilizações contra células cancerígenas específicas e as vantagens e desvantagens do seu uso. Método: Selecionar artigos com estudos sobre nanopartículas entre os anos de 2010 a 2018 nos bancos de dados PubMed e SciELO por meio de revisão integrativa da literatura. Resultados: As nanopartículas apresentam diferentes mecanismos de ação e podem obter resultados mais favoráveis de acordo com sua estrutura química. Conclusão: Concluiu-se com esta revisão que determinadas nanopartículas apresentam funcionalidades que potencializam os efeitos anticancerígenos dos fármacos antineoplásicos e surgem como uma nova abordagem para tratamentos contra o câncer.

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“…Zinc can induce the apoptosis of PC-3 prostate cancer cells [26]. Zinc oxide nanoparticles provide potential anticancer activity toward tongue cancer cells [27]. However, changes in the levels of the trace element zinc in the blood and tissues of patients with osteosarcoma and the regulatory effect of zinc on osteosarcoma cells have not been reported.…”

Section: Discussionmentioning

confidence: 99%

Zinc promotes cell apoptosis via activating the Wnt-3a/β-catenin signaling pathway in osteosarcoma

Gao

Zhang²,

Niu

et al. 2020

J Orthop Surg Res

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Background: The zinc content in the blood and tumor tissues of patients with osteosarcoma and the underlying regulation and molecular mechanism of zinc have not been reported.Methods and results: This study showed that the zinc content in the blood and tumor tissues of patients with osteosarcoma significantly reduced. CCK-8 and Transwell chamber assays revealed that zinc treatment significantly inhibited the proliferation and invasion abilities of osteosarcoma cells. Western blot analysis indicated that the expression levels of caspase-3 and caspase-9 were significantly increased, suggesting that zinc inhibited the growth and promoted the apoptosis of osteosarcoma cells. In addition, the expression levels of Wnt-3a and β-catenin, the marker proteins of the Wnt/β-catenin signaling pathways, were significantly increased in osteosarcoma cells after zinc intervention, which demonstrated that the pathway was clearly activated. However, the effect of zinc on the apoptosis, proliferation, and invasion abilities of osteosarcoma cells was reversed when the Wnt/β-catenin signaling pathways was inhibited by XAV939 (Wnt antagonist) treatment.Conclusions: This study is the first to report the changes in zinc levels in the blood and tumor tissues of patients with osteosarcoma and to preliminarily verify that zinc inhibits the proliferation and invasion and promote the apoptosis of osteosarcoma cells by inducing the Wnt/β-catenin signaling pathway, which ultimately inhibit cancer growth.

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Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy

Cited by 94 publications

References 37 publications

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

The Current Understanding of Autophagy in Nanomaterial Toxicity and Its Implementation in Safety Assessment-Related Alternative Testing Strategies

Utilização de Nanopartículas no Tratamento do Câncer: Aspectos Gerais, Mecanismos de Ação Antineoplásicos e Aplicabilidades Tumorais

Zinc promotes cell apoptosis via activating the Wnt-3a/β-catenin signaling pathway in osteosarcoma

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