We report on the
synthesis and characterization of a novel hybrid
nanoflower of manganese and l-arabinose isomerase and its
application in synthesis of d-tagatose, a rare sugar of high
commercial value. An open reading frame of 1425 base pairs from Lactobacillus sakai was used to synthesis recombinant l-arabinose isomerase of 474 amino acids in E.
coli. A hierarchical flower-like spherical structure
with several nanopetals was self-assembled by using purified recombinant l-arabinose isomerase as the organic component and manganese
phosphate as the inorganic component. The hybrid nanoflower was characterized
by scanning electron microscopy, high-resolution transmission electron
microscopy, confocal laser scanning microscopy, Fourier-transform
infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray
spectroscopy. Circular dichroism documented no change in structural
properties of l-arabinose isomerase assembled in the hybrid
nanoflower. Kinetic parameters of l-arabinose isomerase were
improved in the hybrid nanoflower. l-Arabinose isomerase
in the manganese hybrid nanoflower was found to convert d-galactose to d-tagatose with a conversion rate of ∼50%,
without an addition of manganese in the reaction mixture. The hybrid
nanoflowers exhibited excellent reusability and reproducibility during
reaction cycle analysis. Hence, the developed manganese hybrid nanoflowers
of l-arabinose isomerase shows promise for commercial production
of the rare sugar d-tagatose.
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