Development of nanobiocatalysts through the immobilization of Pseudomonas fluorescens lipase for applications in efficient kinetic resolution of racemic compounds

Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate

“…Also, hydrophobic surface of carbon nanobers act as a catalyst for interfacial activation of lipase resulting into drastic increase in activity aer immobilization. 21,28,29 A comparative analysis showed the precedence of covalent immobilization over adsorption in improving the lipase activity and enzyme loading. Thus, further experiments were carved to optimize other process variables impacting the immobilization through covalent attachment.…”

“…have been extensively used for immobilization of enzymes as they provide cavities adequate for this purpose. [21][22][23] Thus immobilization of enzymes on such support materials can modify their catalytic properties. 4 However, the morphology, distribution pattern of the pores and chemical groups present on the surface of these support materials also affect the enzyme activity.…”

Kinetic resolution of (RS)-1-chloro-3-(4-(2-methoxyethyl)phenoxy)propan-2-ol: a metoprolol intermediate and its validation through homology model of Pseudomonas fluorescens lipase

“…4,5 Lipases as biocatalysts have earned a distinguished position in the eld of chiral synthesis due to their excellent chemo-, regio-and enantioselectivity. 6 Lipases obtained from different sources belong to the a/b-hydrolase folding group. Specic sequences of the a-helices and b-strands are responsible for the hydrolyzing activity of lipases.…”

Exploration of the expeditious potential of Pseudomonas fluorescens lipase in the kinetic resolution of racemic intermediates and its validation through molecular docking

“…PFL has successfully been applied in the enantiopure synthesis of intermediates of important beta blocker drugs like propanolol, atenolol, and CNS drug intermediates like 3‐hydroxymethyl‐1‐tetralone tosylates antifungal N ‐substituted benzimidazole derivatives and has also been used at an industrial scale for the synthesis of Carbovir, an antiviral agent . Several additions to the biocatalytic toolbox of PFL have been made by immobilization of PFL on various matrices like nanoscaffolds, polymeric supports, and hydrophobic sol‐gels …”

“…26 Several additions to the biocatalytic toolbox of PFL have been made by immobilization of PFL on various matrices like nanoscaffolds, polymeric supports, and hydrophobic sol-gels. 5,8 β-Aryloxyalcohols are key intermediates in the synthesis of many important drugs. 30 It has been well established that the desirable therapeutic activities of β-aryloxyalcohols reside mainly in the (S)-enantiomers.…”