Background
Bostrycin is a natural pigment product with anthraquinone skeleton, which has excellent inhibitory effect on a variety cancer cells. However, its suppression on tongue squamous cell carcinoma has not been reported.
Methods
We studied the effects of bostrycin on the cell growth and apoptosis of tongue squamous cell carcinoma
in vitro
, and explored its mechanism.
Results
Bostrycin could inhibit the proliferation and migration of tongue squamous cell carcinoma by arresting cell cycle at G2/M phase, and inducing cell apoptosis with mitochondrial membrane potential changes. West-blotting analysis also showed that bostrycin could inhibit the growth of tongue squamous cell carcinoma cells by activating the apoptosis-related signal proteins especially in mitochondrial apoptotic pathway.
Conclusions
Bostrycin can be used as a new anti-tumor candidate drug for further research. Our study provides reference for the potential application of bostrycin in the treatment of the tongue squamous cell carcinoma.
Background
Intracellular bacteria, especially Mycobacterium tuberculosis, are important pathogenic microorganisms that endanger human health. Purified and synthesized cecropin A‐magainin 2 (CAMA‐syn) can exhibit a higher antibacterial activity and lower cytotoxicity. To enhance such antimicrobial potential, it would be desirable to deliver CAMA‐syn expressed in lung epithelial cells by an adenovirus vector using gene therapy.
Methods
A549 cells in vitro and lung epithelial cells in vivo were used to express CAMA‐syn by transducing recombinant adenovirus Ad‐SPC‐CAMA/GFP, and the expression of CAMA‐syn was determined by a reverse transcriptase‐polymerase reaction (RT‐PCR) and immunofluorescence. The antimicrobial activity in cells was investigated by colony‐forming rate and growth curve. Forty Kunming mice of a Bacillus Calmette‐Guerin (BCG) infection animal model were randomly divided into three groups: adenoviruses delivery of Ad‐SPC‐CAMA/GFP, Ad‐CMV‐CAMA/GFP and empty‐virus Ad‐CMV‐GFP. The expression of CAMA‐syn in mice was confirmed by RT‐PCR and immunofluorescence. After tracheal injection of adenoviral vector for 3 days, lungs from the mouse model were extracted and homogenized for detection of colony‐forming efficiency.
Results
CAMA‐syn expressed in lung epithelial cells A549 conferred antimicrobial activity against a series of bacteria, including Salmonella abortusovis and BCG. The results obtained in vivo showed that the colony‐forming rate of Ad‐SPC‐CAMA/GFP (74.54%) and Ad‐CMV‐CAMA/GFP (62.31%) transduced into mice was significantly lower than that of the control group.
Conclusions
Lung epithelial‐specific expression of antimicrobial peptide CAMA‐syn mediated by adenovirus suppressed the growth of intracellular bacteria, providing a promising approach for the control of refractory intracellular infection.
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