The application of controlled release urea (CRU) has been proposed as a crucial method to reduce the adverse environmental effects induced by conventional urea (CU). Yet, a systematic and quantitative analysis on how CRU affects staple crop production including wheat ( Triticum aestivum L .), maize ( Zea mays L .), and rice ( Oryza sativa L .) is lacking. Here, a meta-analysis was conducted to determine how CRU influences soil chemical properties, total nitrogen (TN) uptake, grain yield, and nitrogen use efficiency (NUE) of staple crop in China. The results indicated that CRU application significantly increased soil organic carbon (SOC), TN, and available nitrogen (AN) by 5.93%, 3.89% and 13.98% respectively overall, while soil pH showed no significant changes. Compared to the application of CU, applying CRU significantly increased grain yield by 7.23%, which was mainly owing to the higher TN uptake (9.13%) across all the studies. In addition, the application of CRU significantly increased NUE, nitrogen agronomy efficiency (NAE), utilization rate of nitrogen fertilizer (NUR), and nitrogen physiological efficiency (NPE) by an average of 23.4%, 34.65%, 25.83% and 15.8% respectively which could be attributed to the slow nitrogen (N) release characteristics of CRU. The positive effect of CRU on grain yield and NUE of staple crop was greatest when the content of SOC and TN were extremely low, indicating that it was most effective to improve grain production of infertile soil by applying CRU. The finding of this study indicated that the application of CRU should be promoted for grain production, especially for infertile soil.

As a fundamental part of the soil ecosystem, prokaryotes are involved in the preservation of soil functions. However, little is known of how the combined application of long-term organic and inorganic nitrogen fertilizer affects the prokaryotic communities’ dynamics at a paddy field. A long-term positioning experiment initiated in 2013 with four treatments (NO: no N fertilizer, CN: 100% urea N with no organic fertilizer, PM: 80% urea N plus 20% N with pig manure, CM: 80% urea N plus 20% N with compost) were applied to detect the differential responses of soil physicochemical properties, and prokaryotic community structure and composition in different fertilization regimes. The results indicated that the long-term combined application of organic and inorganic nitrogen fertilizers altered the physicochemical properties to some extent and, simultaneously, established unique prokaryotic communities. In detail, the treatment of PM and CM significantly increased the content of soil organic carbon (SOC) and total nitrogen (TN) compared to NO. Moreover, a total of 31 indicator taxa were screened across the four treatments by LDA Effect Size (LEfSe) analysis following the principle of the greatest differences, which suggests that these indicator taxa were more sensitive to the fertilization. This research suggested that the combined application of long-term organic and inorganic nitrogen fertilizers not only contributed to the soil’s physicochemical properties but also changed the prokaryotic community composition.

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