Objective: The aim of this study was to quantify the copies of circulating nucleophosmin (NPM) mutations DNA in the plasma of patients with acute myeloid leukemia (AML) and to explore the association of circulating NPM mutation levels with clinical characteristics.Design and Methods: The presence of NPM mutations in 100 Chinese patients newly diagnosed with AML were identified by RT-PCR and sequencing analysis. Copies of circulating NPM mutation A (NPM mut.A) DNA in the plasma of mutation-positive cases were quantified by real-time quantitative PCR (qRT-PCR). Furthermore, the association of circulating NPM mutation levels and clinical characteristics was analyzed.Results: NPM mutations were identified in 37 of the 100 patients and all cases were NPM mut.A. The circulating NPM mut.A levels ranged from 0.35×108 copies/ml to 6.0×108 copies/ml in the 37 mutation-positive cases. The medium and quartile M (P25, P75) of the circulating NPM mut.A levels in patients classified as M2, M4 and M5 morphological subtypes were 1.35×108 (0.76×108, 1.91×108) copies/ml, 1.81×108 (1.47×108, 2.2×108) copies/ml and 2.50×108 (2.42×108, 3.05×108) copies/ml, respectively. Circulating NPM mut.A levels were significantly higher in patients with the M5 subtype of AML compared to patients with the M2 and M4 subtypes (p=0.000, p=0.046). In addition, circulating NPM mut.A copies were significantly associated with a higher white blood cell count, platelet count and bone marrow blast percentage (p<0.05).Conclusion: Our results suggest that circulating NPM mutations DNA assay serves as a complementary to the routine investigative protocol of NPM-mutated leukemia.
Aims: This study aimed to evaluate the different abilities of various Phellinus igniarius strains inhibiting quorum sensing (QS), to search for novel QS inhibitors from them and to analyze their inhibitory activity, with a view to their possible use in controlling infections. Methods: The bioactive metabolites produced by P. igniarius cultures were tested for their abilities to inhibit QS-regulated behavior. All P. igniarius strains were cultured in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2-fold volumes by freeze drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. Results: The bioactive metabolites produced by 10 P. igniarius strains could inhibit violacein production, a QS-regulated behavior in C. violaceum. Furthermore, these strains could be roughly categorized into three groups on the basis of their inhibitory activities. Conclusions:P. igniarius strains can produce QS-inhibitory compounds and have different abilities to inhibit QS.
Aims: This study aimed to search for novel quorum sensing (QS) inhibitors from mushroom and to analyze their inhibitory activity, with a view to their possible use in controlling detrimental infections. Methods: The bioactive metabolites produced by mushroom cultivation were tested for their abilities to inhibit QS-regulated behavior. All mushroom strains were cultivated in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2 vol by freeze-drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. Results: The results have revealed that, of 102 mushroom strains, the bioactive metabolites produced by 14 basidiomycetes were found to inhibit violacein production, a QS-regulated behavior in C. violaceum. Conclusions: Higher fungi can produce QS-inhibitory compounds.
This paper systematically describes the current research methods for analyzing unsteady flows in an open channel. In particular, it focuses on elaborating a discrete method to solve the one-dimensional Saint–Venant equations for unsteady flow in an open channel, compares the advantages and disadvantages of different discrete methods, and proposes a new implicit iterative mathematical model applicable to the calculation of the multiwavelength unsteady flow in the Yangtze River from Yibin to Chongqing. This paper also simulates three typical unsteady flow processes and analyzes the effect of sand excavation on the water level in the upper reaches of the Yangtze River from Yibin to Chongqing considering flood, normal-water, and low-water conditions. The spreading characteristics of the daily adjusted unsteady flow were obtained in the Shuifu-Chongqing river reach. Under the conditions of the daily minimum unsteady discharge flow rate, the designed water level of the reach from Yibin to Chongqing increases by 0.19 m compared to the natural water level. With the increase in concentrated sand excavation in the upper reaches of the Yangtze River, a large number of shingle beaches have disappeared, and the riverbed has been drastically lowered. Affected by the excessive and unordered sand excavation in the river channel, the water level of the upper reaches of the Yangtze River from Chongqing to Yibin has decreased by 0.5–2.0 m.
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