Evaluation of Anti-Quorum-Sensing Activity of Fermentation Metabolites from Different Strains of a Medicinal Mushroom, Phellinus igniarius

Abstract

Aims: This study aimed to evaluate the different abilities of various Phellinus igniarius strains inhibiting quorum sensing (QS), to search for novel QS inhibitors from them and to analyze their inhibitory activity, with a view to their possible use in controlling infections. Methods: The bioactive metabolites produced by P. igniarius cultures were tested for their abilities to inhibit QS-regulated behavior. All P. igniarius strains were cultured in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2-fold volumes by freeze drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. Results: The bioactive metabolites produced by 10 P. igniarius strains could inhibit violacein production, a QS-regulated behavior in C. violaceum. Furthermore, these strains could be roughly categorized into three groups on the basis of their inhibitory activities. Conclusions:P. igniarius strains can produce QS-inhibitory compounds and have different abilities to inhibit QS.

Fungal Diversity in Intertidal Mudflats and Abandoned Solar Salterns as a Source for Biological Resources

“…Some fungi produce quorum-quenching compounds such as patulin and penicillic acid from Penicillium spp., which can suppress bacterial biofilm formation [68]. Other studies reported that the extracts of Fusarium graminearum , Lasidiplodia sp., Phellinus igniarius , and fruiting bodies of Auricularia auricular , Tremella fuciformis , and Ganoderma lucidum have QS inhibitory activity [69,70,71,72,73,74]. Fungal QSIs were reported in the genera Penicillium and Trichoderma : polyhydroxyanthraquinones, penicillic acid, and patulin from P. restrictum , P. radicicola , and P. coprobium , respectively [68,75]; and carot-4-en-9,10-diol from T. virens [76].…”

Quorum Sensing Inhibition by Asparagopsis taxiformis, a Marine Macro Alga: Separation of the Compound that Interrupts Bacterial Communication

“…The C. violaceum CV026 is a mutant strain incapable of producing AHL and violacein. It is a versatile and easy-to-use reporter that responds to exogenous AHLs and is widely used in quorum sensing inhibition assay [21,22,23,24]. The strain is also used for checking antibacterial activity [25].…”

Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa

“…The only reports so far of possible anti-QS activity are from two plant root-associated fungi, i.e. , Phialocephala fortinii and Meliniomyces variabilis [23] that are able to interfere with the N-acylated homoserine lactone regulatory system of several Gram-negative bacteria, and of Phellinus igniarius [24] and Ganoderma lucidum [25] that can suppress violacein production of Chromobacter violaceum .…”