A simple portable continuous L-lactate monitoring system combining elegant ultrafiltration sample collection and storage with a lactate biosensor has been developed. The sensor has a detection range from 0.05mM to 30mM 2153 Downloaded by [Temple University Libraries] at 20:54 13 June 2016 2154 CHENG ETAL. lactate dependent on different assignments of the PCS hydrogel complex layer. The sensor shows excellent performance on sensitivity and validity as well as operational stability over one month. Samples containing different concentrations of lactate were collected in six hours with an ultrafiltration probe and stored in a 6m long PEEK tube of 125jxm in internal diameter. The ultraslow flow rate of 100 to 350nl/min was controlled by adjusting the restriction in the portable plastic syringe pump and a constant flow can be maintained over 24 hours. The collected samples were then detected after reversing the flow with a lactate sensor. The total weight of the collection system is only 8g, allowing free movement of the person being tested.
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